Document Detail


A thrombin-like enzyme from bushmaster (Lachesis muta stenophyrs) venom.
MedLine Citation:
PMID:  8310444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The clotting enzyme (Stenoxobin), from the venom of Lachesis muta stenophyrs, was purified by gel chromatography on Bio-gel P-100 followed by agmatine CH-Sepharose-4B and FPLC on Mono Q column. By SDS polyacrylamide gel electrophoresis the mol. wt was found to be 37,000. The enzyme is a glycoprotein with 1.6 moles of sialic acid per mole of protein and has an average content of 7.0% of neutral carbohydrates. The clotting and esterolytic (BAEE) activities were 843 NIH units/mg and 60.1 +/- 1.2 OD225 ml/min/mg, respectively, and could not be inhibited by heparin or hirudin. Amino acid analysis revealed a low content of tryptophan and a high content of acid residues. Stenoxobin acts upon human fibrinogen by releasing consecutively fibrinopeptides A and B from the alpha- and beta-chains of fibrinogen.
Authors:
F Aragon-Ortiz; F Gubensek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  31     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-03-17     Completed Date:  1994-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1435-43     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, School of Medicine, University of Costa Rica, San Jose.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coagulants / chemistry,  isolation & purification*,  pharmacology
Crotalid Venoms / enzymology*
Electrophoresis, Polyacrylamide Gel
Glycoproteins / chemistry,  isolation & purification*,  pharmacology
Thrombin / pharmacology
Chemical
Reg. No./Substance:
0/Coagulants; 0/Crotalid Venoms; 0/Glycoproteins; 154303-71-6/stenoxobin protein, Lachesis muta stenophyrs; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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