Document Detail

A thrombin-like enzyme from bushmaster (Lachesis muta stenophyrs) venom.
MedLine Citation:
PMID:  8310444     Owner:  NLM     Status:  MEDLINE    
The clotting enzyme (Stenoxobin), from the venom of Lachesis muta stenophyrs, was purified by gel chromatography on Bio-gel P-100 followed by agmatine CH-Sepharose-4B and FPLC on Mono Q column. By SDS polyacrylamide gel electrophoresis the mol. wt was found to be 37,000. The enzyme is a glycoprotein with 1.6 moles of sialic acid per mole of protein and has an average content of 7.0% of neutral carbohydrates. The clotting and esterolytic (BAEE) activities were 843 NIH units/mg and 60.1 +/- 1.2 OD225 ml/min/mg, respectively, and could not be inhibited by heparin or hirudin. Amino acid analysis revealed a low content of tryptophan and a high content of acid residues. Stenoxobin acts upon human fibrinogen by releasing consecutively fibrinopeptides A and B from the alpha- and beta-chains of fibrinogen.
F Aragon-Ortiz; F Gubensek
Related Documents :
9197404 - Isolation and partial characterization of a fatty-acid-binding protein from ascaris suu...
6462174 - Major high mobility group like proteins of drosophila melanogaster embryonic nuclei.
12833504 - Soft immobilized ph gradient gels in proteome analysis: a follow-up.
6667924 - Electrophoretic investigations of acid-stable proteinase-inhibitory activity in human s...
6790514 - Dihydroxy and monohydroxy fatty acids in legionella pneumophila.
7275864 - Influence of captan on in vitro and in vivo digestibility of forage.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  31     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-03-17     Completed Date:  1994-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1435-43     Citation Subset:  IM    
Department of Biochemistry, School of Medicine, University of Costa Rica, San Jose.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Coagulants / chemistry,  isolation & purification*,  pharmacology
Crotalid Venoms / enzymology*
Electrophoresis, Polyacrylamide Gel
Glycoproteins / chemistry,  isolation & purification*,  pharmacology
Thrombin / pharmacology
Reg. No./Substance:
0/Coagulants; 0/Crotalid Venoms; 0/Glycoproteins; 154303-71-6/stenoxobin protein, Lachesis muta stenophyrs; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Disulfide formation in reduced tetanus toxin by thioredoxin: the pharmacological role of interchain ...
Next Document:  Coagulation factor X inhibitor from hundred-pace snake (Deinagkistrodon acutus) venom.