| A threshold level of gag expression is required for particle formation in rat cells transformed by avian retroviruses. | |
| | |
MedLine Citation:
|
PMID: 10732719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To test the hypothesis that the block of polyprotein precursor processing and particle formation in RSV-transformed mammalian cells is due to a low level of pr76gag expression, rat tumor cell lines with different amounts of precursor molecules were used. The wild-type forms of pr76gag have been expressed at a high level by use of SV40-based vector and thirty-two stable transfected cell clones were isolated. The gag protein expression was detected in the cell lysate by immunoblotting. Untransfected cells released no proteins that could be detected by immunoprecipitation with anti-RSV serum. Membrane-enclosed gag precursor-polyprotein molecules and infectious virus particles from different stably transfected clones have been found in the medium. Both immature and mature virions of type C morphology were directly detected by transmission electron microscopy. Surprisingly, virus-like particles of morphology similar to mature type C retroviruses were found enclosed within intracellular membranes in a stably transfected nonproducing clone. |
| | |
Authors:
|
M Polianova; M Alexandrov; V Sovová; K Simeonov; R Roussev; D Beshkov; T G Todorov |
Related Documents
:
|
18597389 - Recombinant protein excretion in escherichia coli jm103[puc8]: effects of plasmid conte... 1737639 - Enhanced transfection of a bacterial plasmid into hybridoma cells by electroporation: a... 10085259 - Polarized targeting of epithelial cell proteins in thyrocytes and mdck cells. 18455459 - Functional assay of the mutant tissue-nonspecific alkaline phosphatase gene using u2os ... 3061379 - Constitutive and inducible expression of cloned human interferon-b1 gene in hela cells ... 18599879 - Work flow for multiplexing sirna assays by solid-phase reverse transfection in multiwel... 3013119 - Breast carcinoma with osteoclastlike giant cells. 8613679 - Increased micronucleus frequency in the progeny of irradiated chinese hamster cells. 22552889 - Role of a dna damage checkpoint pathway in ionizing radiation-induced glioblastoma cell... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Folia biologica Volume: 45 ISSN: 0015-5500 ISO Abbreviation: Folia Biol. (Praha) Publication Date: 1999 |
Date Detail:
|
Created Date: 2000-04-24 Completed Date: 2000-04-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0234640 Medline TA: Folia Biol (Praha) Country: CZECH REPUBLIC |
Other Details:
|
Languages: eng Pagination: 233-41 Citation Subset: IM; X |
Affiliation:
|
National Reference AIDS Laboratory, National Center of Infections and Parasitic Diseases, Sofia, Bulgaria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Avian Sarcoma Viruses / genetics, isolation & purification, physiology*, ultrastructure Cell Line, Transformed Cell Transformation, Viral* Gene Expression Regulation, Viral* Gene Products, gag / biosynthesis*, genetics Genes, gag* Rats Sarcoma, Experimental / pathology Transfection Tumor Cells, Cultured Virion / metabolism*, ultrastructure Virus Replication* |
| Chemical | |
Reg. No./Substance:
|
0/Gene Products, gag |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interleukin 2 gene therapy of surgical minimal residual tumour disease: characterization of cytolyti...
Next Document: The asymmetric distribution of interphasic silver-stained nucleolus organizer regions in human and r...