Document Detail


The threshold for immune cell reactivity to milk antigens decreases in cow's milk allergy with intestinal symptoms.
MedLine Citation:
PMID:  8876554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In cow's milk allergy (CMA) with intestinal symptoms, peripheral blood mononuclear cells (PBMCs) secrete tumor necrosis factor-alpha (TNF-alpha), altering intestinal function. However, the type of cow's milk protein (CMP) that triggers symptoms (intact or intestinally processed) is not known, and neither is the minimal amount required. METHODS: PBMCs were isolated from infants with active CMA or cured infants just before a new challenge and stimulated with intact or intestinally processed CMP. Supernatants were tested for cytokine content and for their ability to perturb intestinal barrier capacity, measured in Using chambers in HT29-19A intestinal cells. RESULTS: PBMCs from infants with active CMA secreted more TNF-alpha, when they were stimulated with intact rather than intestinally processed CMPs, and more TNF-alpha than PBMCs from cured infants. Accordingly, supernatants from PBMCs stimulated with intact but not intestinally processed CMPs significantly increased intestinal permeability. The CMP concentration required to trigger TNF-alpha secretion capable of altering intestinal function was very small in infants with active CMA (approximately 2 micrograms/ml), but about 300 times higher in cured infants. CONCLUSION: Intact rather than intestinally processed proteins stimulate PBMCs to release TNF-alpha and alter intestinal barrier capacity. The threshold for PBMC reactivity to milk antigens drops considerably during active CMA with intestinal symptoms.
Authors:
N Benlounes; C Dupont; C Candalh; M A Blaton; N Darmon; J F Desjeux; M Heyman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  98     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-27     Completed Date:  1996-11-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  781-9     Citation Subset:  AIM; IM    
Affiliation:
Institut National de la Santé et de la Recherche Médicale (U290), Hôpital St. Lazare, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cytokines / metabolism
Dose-Response Relationship, Immunologic
Humans
Immunity, Cellular
Immunity, Mucosal
Infant
Intestinal Absorption
Leukocytes, Mononuclear / immunology*
Milk Hypersensitivity / immunology*
Milk Proteins / chemistry,  immunology*
Peptide Fragments / immunology
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / secretion*
Chemical
Reg. No./Substance:
0/Cytokines; 0/Milk Proteins; 0/Peptide Fragments; 0/Tumor Necrosis Factor-alpha

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