Document Detail


A three-dimensional collagen matrix as a suitable culture system for the comparison of cyclic strain and hydrostatic pressure effects on intervertebral disc cells.
MedLine Citation:
PMID:  15871486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECT: To study intervertebral disc cell mechanobiology, the authors developed experimental systems that allow the application of cyclic strain and intermittent hydrostatic pressure (IHP) on isolated disc cells under equal three-dimensional (3D) culture conditions. The purpose of the study was to characterize disc cell proliferation, viability, morphology, and gene expression in 3D collagen matrices. METHODS: The effects of cyclic strain (1, 2, 4, and 8% strain; 1 Hz) and IHP (0.25 MPa, 0.1 Hz) on gene expression (real-time polymerase chain reaction) of anabolic and catabolic matrix proteins were investigated and compared with those derived from mechanically unstimulated controls. Intervertebral disc cells proliferated in the collagen gels (mean viability 91.6%) and expressed messenger RNA for collagen I, collagen II, aggrecan, matrix metalloproteinase (MMP)-2, and MMP-3. Morphologically, both spindle-shaped cells with longer processes and rounded cells were detected in the collagen scaffolds. Cyclic strain increased collagen II and aggrecan expression and decreased MMP-3 expression of anulus fibrosus cells. No significant difference between the four strain magnitudes was found. Intermittent hydrostatic pressure tended to increase collagen I and aggrecan expression of nucleus cells and significantly decreased MMP-2 and -3 expression of nucleus cells, whereas aggrecan expression of anulus cells tended to decrease. CONCLUSIONS: Based on these results, the collagen matrix appeared to be a suitable substrate to apply both cyclic strain and IHP to intervertebral disc cells under 3D culture conditions. Individual variations may be influenced by the extent of degeneration of the disc specimens from which the cells were isolated. This experimental setup may be suitable for studying the influence of degeneration on the disc cell response to mechanical stimuli.
Authors:
Cornelia Neidlinger-Wilke; Karin Würtz; Astrid Liedert; Carla Schmidt; Wolfgang Börm; Anita Ignatius; Hans-Joachim Wilke; Lutz Claes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurosurgery. Spine     Volume:  2     ISSN:  1547-5654     ISO Abbreviation:  -     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-05     Completed Date:  2005-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101223545     Medline TA:  J Neurosurg Spine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  457-65     Citation Subset:  IM    
Affiliation:
Institute of Orthopaedic Research and Biomechanics, University of Ulm, Germany. cornelia.neidlinger-wilke@medizin.uni-ulm.de
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MeSH Terms
Descriptor/Qualifier:
Aggrecans
Biomechanics
Biopsy
Cell Movement
Collagen Type I / biosynthesis*,  genetics
Collagen Type II / biosynthesis*,  genetics
DNA Primers / genetics
Equipment Design
Extracellular Matrix / genetics,  metabolism*
Extracellular Matrix Proteins / genetics,  metabolism
Gene Expression / genetics
Humans
Hydrostatic Pressure
Intervertebral Disk / metabolism*,  pathology
Intervertebral Disk Displacement / metabolism*,  pathology
Lectins, C-Type
Matrix Metalloproteinase 2 / genetics,  metabolism
Matrix Metalloproteinase 3 / genetics,  metabolism
Periodicity
Polymerase Chain Reaction / instrumentation*
Proteoglycans / genetics,  metabolism
RNA, Messenger / genetics
Chemical
Reg. No./Substance:
0/Aggrecans; 0/Collagen Type I; 0/Collagen Type II; 0/DNA Primers; 0/Extracellular Matrix Proteins; 0/Lectins, C-Type; 0/Proteoglycans; 0/RNA, Messenger; EC 3.4.24.17/Matrix Metalloproteinase 3; EC 3.4.24.24/Matrix Metalloproteinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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