Document Detail

A thin glycoprotein coating of a synthetic lenticule does not cause nutritional deficiency of the anterior cornea.
MedLine Citation:
PMID:  10372994     Owner:  NLM     Status:  MEDLINE    
PURPOSE: This study investigated whether a glycoprotein coating that will be used to enhance corneal epithelialization affects in situ nutritional passage through a permeable membrane. METHODS: Sixteen adult cats were equally divided into two groups. Polycarbonate membranes with pore size of 0.1 microm and total pore area (porosity) of 3.1% were used as implant materials. The membranes for Group 1 were coated with a thin layer of Collagen I, while the membranes for Group 2 were uncoated. Each membrane with 8-mm diameter was implanted into an interlamellar pocket of the cornea. The eyes were observed for approximately 35 days to monitor clinical signs of nutritional deficiency of the cornea, and then 7 membranes were removed from the eyes. The permeability of the explanted membranes to glucose, inulin and albumin was used to predict the in situ difference between the coated and uncoated groups in regard to nutritional passage through the membranes. To investigate the long-term effect of the surface coating on corneal health, two animals from Group 1 were followed for up to two years and then both eyes of each animal underwent histological examination. RESULTS: Clinically, no post-surgical complications associated with nutritional deficiency were observed in any of the eyes. Nutritional permeability tests showed no significant differences between the coated and uncoated membranes. Histologically, the long-term animals showed no abnormal morphology associated with nutritional deficiency in the cornea anterior or posterior to the membranes. CONCLUSIONS: A thin glycoprotein coating on a permeable membrane does not appear to affect the nutritional supply of the anterior cornea and therefore can be used to enhance epithelialization of synthetic corneal onlays in vivo.
R Z Xie; D F Sweeney; H J Griesser; S Tout; H Y Cheng; J G Steele
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current eye research     Volume:  18     ISSN:  0271-3683     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-07-27     Completed Date:  1999-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  335-41     Citation Subset:  IM    
Cooperative Research Centre for Eye Research and Technology, University of New South Wales, Sydney, Australia.
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MeSH Terms
Cornea / pathology,  physiopathology*,  surgery*
Membranes, Artificial*
Prostheses and Implants*
Reference Values
Surface Properties
Reg. No./Substance:
0/Glycoproteins; 0/Membranes, Artificial

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