Document Detail


Is there a synergistic effect between mixed bacterial infection in biofilm formation on biliary stents?
MedLine Citation:
PMID:  9744599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Biliary sludge which forms as a result of bacterial adherence and biofilm formation in the biliary system is a recognized cause of blockage of plastic stents. Bacteriological cultures of sludge have revealed a mixed infection with gram-positive and gram-negative bacteria. Animal studies have shown that prophylactic ciprofloxacin, which selectively suppress gram-negative bacteria, results in prolonged stent patency despite colonization of the stents by gram-positive bacteria. METHODS: We tested a possible synergistic effect between gram-negative and gram-positive bacteria in adherence and biofilm formation on plastic stents. Clinical isolates of Escherichia coli and Enterococcus were cultured in separate chemostats to achieve a steady growth. Adherence of the two bacteria on plastic stent surface were tested separately by perfusing infected bile with the respective bacteria through different modified Robbins devices containing 10F polyethylene stent pieces up to 4 days. In a second experiment, Enterococcus was perfused through stent pieces precolonized with E. coli for 24 hours. The stent pieces were then removed daily and analyzed by bacteriologic culture and scanning electron microscopy for bacterial adherence and biofilm formation. RESULTS: Gram-negative E. coli were more adherent than gram-positive Enterococcus. Precolonization with E. coli facilitates subsequent attachment of Enterococcus. CONCLUSIONS: We concluded that there is a synergistic effect between gram-positive and gram-negative bacteria in adherence and biofilm formation.
Authors:
J W Leung; Y L Liu; T Desta; E Libby; J F Inciardi; K Lam
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Gastrointestinal endoscopy     Volume:  48     ISSN:  0016-5107     ISO Abbreviation:  Gastrointest. Endosc.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-10     Completed Date:  1998-12-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0010505     Medline TA:  Gastrointest Endosc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  250-7     Citation Subset:  IM    
Affiliation:
Division of Gastroenterology and C. W. Law Biofilm Research Center, University of California, Davis Medical Center, Sacramento 95817, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / administration & dosage
Bacterial Adhesion*
Biliary Tract Surgical Procedures / instrumentation
Biofilms*
Cattle
Colony-Forming Units Assay
Enterococcus / physiology,  ultrastructure*
Equipment Contamination
Escherichia coli / physiology,  ultrastructure*
Gram-Negative Bacterial Infections / prevention & control
Gram-Positive Bacterial Infections / prevention & control
Humans
Microbiological Techniques
Microscopy, Electron, Scanning
Middle Aged
Species Specificity
Stents / microbiology*
Surface Properties
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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