| Is there a role for antioxidants in prevention of pulmonary hypoplasia in nitrofen-induced rat model of congenital diaphragmatic hernia? | |
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MedLine Citation:
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PMID: 20127338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: Many studies suggest a role for antioxidants in the prevention of lung hypoplasia in nitrofen-induced rat models with congenital diaphragmatic hernia (CDH). This study investigates the oxidative status and the histological outcome of prenatal administration of vitamins E and C with synergistic effect, and effect of N-acetylcysteine (NAC) to improve lung maturation of nitrofen-induced rats. METHODS: CDH was induced by maternal administration of a single oral dose of nitrofen on day 9.5 of gestation, and the Sprague-Dawley rats were randomly divided into five groups: nitrofen (N), nitrofen + vitamin C (NC), nitrofen + vitamin E (NE), nitrofen + vitamin C + vitamin E (NCE) and nitrofen + NAC (NNAC). A control group in which only vehicle was administered was included. Cesarean section was performed on day 21. Body weight (BW) and total lung weight (LW) of all fetuses with CDH were recorded; lung histological evaluation was performed, and protein content of lungs, determination of thiobarbituric acid reactive substances, and the protein carbonyls in tissue samples were determined. RESULTS: A total of 133 rat fetuses with CDH were investigated. The body weight and the lung weight of fetuses of all groups that were exposed to nitrofen were significantly decreased than of the control group (P < 0.05). The animals exposed to nitrofen with different antioxidants showed increased protein levels in lung tissue. However, in the NCE and the NNAC groups, protein levels were significantly increased than in the others. Malondialdehyde levels significantly decreased in the NCE and the NNAC groups when compared with the NC and the NE groups. In addition, the NCE and NNAC groups decreased protein oxidation to control levels, and no significant difference was observed between control and these two antioxidants groups. The N, NC, NE and NNAC groups showed minimal improvement in lung histology; the NCE groups showed the most improvement in lung histology when compared with the other nitrofen plus antioxidant groups. CONCLUSION: Prenatal administration of NAC and vitamin E in combination with vitamin C represented the best effects to avoid oxidative damage and protein content of the lungs in rat pups with CDH at birth. |
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Authors:
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Murat Kemal Cigdem; Goksel Kizil; Abdurrahman Onen; Murat Kizil; Yusuf Nergiz; Yusuf Celik |
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Publication Detail:
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Type: Journal Article Date: 2010-02-03 |
Journal Detail:
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Title: Pediatric surgery international Volume: 26 ISSN: 1437-9813 ISO Abbreviation: Pediatr. Surg. Int. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-18 Completed Date: 2010-06-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8609169 Medline TA: Pediatr Surg Int Country: Germany |
Other Details:
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Languages: eng Pagination: 401-6 Citation Subset: IM |
Affiliation:
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Department of Pediatric Surgery, Medical School, Dicle University, 21280, Diyarbakir, Turkey. mkcigdem@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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therapeutic use Animals Antioxidants / therapeutic use* Ascorbic Acid / therapeutic use* Disease Models, Animal Drug Synergism Female Fetus / drug effects Free Radical Scavengers / therapeutic use Hernia, Diaphragmatic / chemically induced, complications*, congenital Lipid Peroxidation / drug effects Lung / drug effects Lung Diseases / prevention & control* Male Maternal-Fetal Exchange Pesticides Phenyl Ethers / administration & dosage Pregnancy Proteins / drug effects, metabolism Rats Rats, Sprague-Dawley Thiobarbituric Acid Reactive Substances / metabolism Vitamin E / therapeutic use* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Free Radical Scavengers; 0/Pesticides; 0/Phenyl Ethers; 0/Proteins; 0/Thiobarbituric Acid Reactive Substances; 1406-18-4/Vitamin E; 1836-75-5/nitrofen; 50-81-7/Ascorbic Acid; 616-91-1/Acetylcysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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