Document Detail


Is there a possibility for a glutaraldehyde-free porcine heart valve to grow?
MedLine Citation:
PMID:  16490995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: A challenging issue is to create a heart valve with growth and remodeling potential, which would be of great interest for congenital heart valve surgery. This study was performed to evaluate the growth and remodeling potentials of a decellularized heart valve. METHODS: In 4 juvenile sheep (age 12 +/- 1 weeks) with a weight of 24.3 +/- 4.4 kg, a 17-mm diameter decellularized porcine valve was implanted as pulmonary valve replacement. Valve growth was evaluated by transthoracic echocardiography. At explantation, valves were evaluated by gross examination, light microscopy (hematoxylin and eosin, von Kossa, Sirius red, Weigert and Gomori staining), electron microscopy and immunohistochemistry. Atomic absorption spectrometry was performed to evaluate calcium content. RESULTS: All animals showed fast recovery. The mean follow-up was 9.0 +/- 1.8 months. All sheep at least doubled their weight (54.3 +/- 9.2 kg). Echocardiography showed no regurgitation and a flow velocity of 0.7 +/- 0.1 m/s at the latest follow-up. The valve diameter increased from 17.6 +/- 0.5 to 27.5 +/- 2.1 mm (p < 0.018). Gross examination showed a similar wall thickness of the implanted valve and native pulmonary wall, with smooth and pliable leaflets. Histology showed a monolayer of endothelial cells, fibroblast ingrowth and production of new collagen. No calcification was seen at von Kossa staining, confirmed by low calcium content levels of the valve wall and leaflets at atomic absorption spectrometry. CONCLUSIONS: This glutaraldehyde-free heart valve showed not only the absence of calcification, but also remodeling and growth potential.
Authors:
P M Dohmen; F da Costa; S Holinski; S V Lopes; S Yoshi; L H Reichert; R Villani; S Posner; W Konertz
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Publication Detail:
Type:  Journal Article     Date:  2006-02-16
Journal Detail:
Title:  European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes     Volume:  38     ISSN:  0014-312X     ISO Abbreviation:  Eur Surg Res     Publication Date:  2006  
Date Detail:
Created Date:  2006-03-14     Completed Date:  2006-05-02     Revised Date:  2006-11-30    
Medline Journal Info:
Nlm Unique ID:  0174752     Medline TA:  Eur Surg Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  54-61     Citation Subset:  IM    
Copyright Information:
Copyright 2006 S. Karger AG, Basel.
Affiliation:
Department of Cardiovascular Surgery, Charité Hospital, Medical University, Berlin, Germany. pascal.dohmen@charite.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Echocardiography, Transesophageal
Glutaral / pharmacology*
Heart Valve Prosthesis*
Heart Valves / drug effects,  growth & development*
Models, Animal
Pulmonary Valve / growth & development*,  transplantation*
Sheep
Swine
Transplantation, Heterologous
Chemical
Reg. No./Substance:
111-30-8/Glutaral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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