Document Detail

Is there a genetic variation association in the IL-10 and TNF alpha promoter gene with gestational diabetes mellitus?
MedLine Citation:
PMID:  20536548     Owner:  NLM     Status:  MEDLINE    
Gestational diabetes mellitus (GDM), defined as carbohydrate intolerance diagnosed for the first time during pregnancy, affects both maternal and fetal health. Possession of a specific genetic polymorphism can be a predisposing factor for susceptibility to some diseases. The aim of this study was to investigate the association between single nucleotide polymorphisms (SNP) in the promoter gene of interleukin-10 (IL-10) as well as tumor necrosis factor-alpha (TNF alpha) with the development of GDM. Two hundred and twelve consecutive series of eligible normal pregnant women (controls) and gestational diabetes mellitus women were selected based on the study's inclusion and exclusion criteria. DNA was extracted from blood and genotyped for IL-10 at three positions and TNF alpha for gene polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma levels of IL-10 and TNF alpha at different gestational periods as well as postpartum were quantified using enzyme linked immunosorbent assay (ELISA). The results of the study showed that the difference in the frequency of SNP at position -597 in the promoter of the human IL-10 gene between the control and GDM groups was statistically significant (p < 0.05). In contrast, there was no significant difference in the frequency of SNP at the other two sites in the promoter region of the human IL-10 gene (-824 and -1082) as well as position -308 in the promoter of the human TNF-alpha (p > 0.05). In addition, there was no significant difference between the two groups in terms of plasma levels of IL-10 as well as TNF alpha in different stages of pregnancy. SNP at position -597 was significantly associated with the development of GDM and shows potential for use as a predictive marker for GDM.
Shabnam Montazeri; Sivalingam Nalliah; Ammu Kutty Radhakrishnan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hereditas     Volume:  147     ISSN:  1601-5223     ISO Abbreviation:  Hereditas     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-06-11     Completed Date:  2010-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374654     Medline TA:  Hereditas     Country:  Sweden    
Other Details:
Languages:  eng     Pagination:  94-102     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Faculty of Medicine and Health, International Medical University (IMU), Bukit Jalil, Kuala Lumpur, Malaysia.
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MeSH Terms
Case-Control Studies
Diabetes, Gestational / genetics*
Genetic Variation*
Interleukin-10 / genetics*
Middle Aged
Promoter Regions, Genetic*
Tumor Necrosis Factor-alpha / genetics*
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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