Document Detail


The tetraspanin BcPls1 is required for appressorium-mediated penetration of Botrytis cinerea into host plant leaves.
MedLine Citation:
PMID:  14731267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Animal tetraspanins are membrane proteins controlling cell adhesion, morphology and motility. In fungi, the tetraspanin MgPls1 controls an appressorial function required for the penetration of Magnaporthe grisea into host plants. An orthologue of MgPLS1, BcPLS1, was identified in the necrotrophic fungal plant pathogen Botrytis cinerea. We constructed a Bcpls1::bar null mutant by targeted gene replacement. Bcpls1::bar is not pathogenic on intact plant tissues of bean, tomato or rose, but it infects wounded plant tissues. Both wild type and Bcpls1::bar differentiate appressoria on plant and artificial surfaces, a process involving an arrest of polarized growth, apex swelling and its cell wall reinforcement. Although wild-type appressoria allowed the penetration of the fungus into the host plant within 6-12 h, no successful penetration events were observed with Bcpls1::bar, suggesting that its appressoria are not functional. An eGFP transcriptional fusion showed that BcPLS1 was specifically expressed in conidia, germ tubes and appressoria during host penetration. Our results indicate that BcPLS1 is required for the penetration of B. cinerea into intact host plants. The defect in pathogenicity of Bcpls1::bar also demonstrates that functional B. cinerea appressoria are required for a successful penetration process. As Bcpls1::bar and Mgpls1 Delta::hph penetration defects are similar, fungal tetraspanins are likely to be required for an essential appressorial function widespread among fungi.
Authors:
M Gourgues; A Brunet-Simon; M-H Lebrun; C Levis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  51     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-20     Completed Date:  2004-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  619-29     Citation Subset:  IM    
Affiliation:
FRE 2739, CNRS/Bayer, Physiologie des Plantes et des Champignons, Bayer Cropscience, 14-20 Rue Pierre Baizet, 69009 Lyon, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ504994
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics,  metabolism*
Botrytis / cytology*,  genetics,  metabolism,  pathogenicity*
Cell Differentiation / physiology
Gene Targeting
Genetic Complementation Test
Humans
Magnaporthe / genetics,  metabolism
Membrane Proteins / genetics,  metabolism*
Molecular Sequence Data
Mutation
Plant Leaves / microbiology*
Recombinant Fusion Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Membrane Proteins; 0/Recombinant Fusion Proteins

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