Document Detail


The testicular and epididymal luminal amino acid microenvironment in the rat.
MedLine Citation:
PMID:  2086576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Concentrations of amino acids were measured in arterial and testicular venous blood, and in fluids from the seminiferous tubule, rete testis, and the caput, corpus, and cauda epididymidis. There were no significant differences in the concentrations of any amino acids between arterial and testicular venous blood, whereas there were significant differences between arterial/venous blood and testicular interstitial fluid. The predominant amino acids measured within seminiferous tubule fluid (STF) and rete testis fluid (RTF) were glycine, alanine, glutamate, and glutamine. RTF contained approximately equal concentrations of basic and total amino acids, but 17 times higher acidic amino acids and 1.2 and 1.3 times lower uncharged polar and nonpolar amino acids, respectively, compared to STF. The concentration of total amino acids within caput fluid reached over 50 nmol/L, but then declined to approximately 50% and 0.1% of caput for corpus and cauda, respectively. The predominant amino acids measured within epididymal luminal fluids were glutamate and taurine; glutamate contributed to approximately 90% of the total amino acids measured in caput fluid. The presence of glutamate and taurine within the epididymal lumen is due primarily to a direct contribution from the epididymal epithelium, as measured using the split-drop stopped-flow microperfusion technique. Several other amino acids within the lumen also originate from the epididymal epithelium. Amino acids contribute approximately 20%, 9%, and 2% of the total osmolality of caput, corpus, and cauda fluid, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
B T Hinton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of andrology     Volume:  11     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    1990 Nov-Dec
Date Detail:
Created Date:  1991-05-20     Completed Date:  1991-05-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  498-505     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, University of Virginia Health Sciences Center, Charlottesville 22908.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*,  secretion
Analysis of Variance
Animals
Epididymis / metabolism*
Extracellular Space / metabolism
Male
Osmolar Concentration
Rats
Rats, Inbred Strains
Testis / metabolism*
Grant Support
ID/Acronym/Agency:
HD-18257/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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