| A test battery for the ecotoxicological evaluation of pentachlorophenol. | |
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MedLine Citation:
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PMID: 11566584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experimental bioassays are currently used in ecotoxicology and environmental toxicology to provide information for risk assessment evaluation of new chemicals and to investigate their effects and mechanisms of action; in addition, ecotoxicological models are used for the detection, control and monitoring of the presence of pollutants in the environment. As a single bioassay will never provide a full picture of the quality of the environment, a representative, cost-effective and quantitative test battery should be developed. The effects of pentachlorophenol were studied using a battery of ecotoxicological model systems, including immobilization of Daphnia magna, bioluminiscence inhibition in the bacterium Vibrio fischeri, growth inhibition of the alga Chlorella vulgaris, and micronuclei induction in the plant Allium cepa. The inhibition of cell proliferation and MTT reduction were investigated in Vero cells. Neutral red uptake, cell growth, MTT reduction, lactate dehydrogenase leakage and activity were studied in the salmonid fish cell line RTG-2, derived from the gonad of rainbow trout. Pentachlorophenol was very toxic for all biota and cells. The system most sensitive to pentachlorophenol, was micronuclei induction in A. cepa, followed by D. magna immobilization, bioluminescence inhibition in V. fischeri bacteria at 60 min and cell proliferation inhibition of RTG-2 cells at 72 h. Inhibition of cell proliferation and MTT reduction on Vero monkey cells showed intermediate sensitivity. |
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Authors:
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G Repetto; A Jos; M J Hazen; M L Molero; A del Peso; M Salguero; P D Castillo; M C Rodríguez-Vicente; M Repetto |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 15 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2001 Aug-Oct |
Date Detail:
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Created Date: 2001-09-21 Completed Date: 2001-12-04 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 503-9 Citation Subset: IM |
Affiliation:
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National Institute of Toxicology, PO Box 863, 41080 Seville, Spain. repetto@sev.inaltox.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cercopithecus aethiops Chlorella / drug effects, growth & development Daphnia / drug effects, physiology Dose-Response Relationship, Drug Ecosystem* Environmental Pollutants / toxicity* L-Lactate Dehydrogenase / metabolism Luminescent Measurements Micronuclei, Chromosome-Defective / drug effects Models, Biological Neutral Red / metabolism Oncorhynchus mykiss Onions / drug effects, genetics Pentachlorophenol / toxicity* Tetrazolium Salts / metabolism Toxicity Tests / methods* Vero Cells Vibrio / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Environmental Pollutants; 0/Tetrazolium Salts; 553-24-2/Neutral Red; 87-86-5/Pentachlorophenol; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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