Document Detail

A test battery for the ecotoxicological evaluation of pentachlorophenol.
MedLine Citation:
PMID:  11566584     Owner:  NLM     Status:  MEDLINE    
Experimental bioassays are currently used in ecotoxicology and environmental toxicology to provide information for risk assessment evaluation of new chemicals and to investigate their effects and mechanisms of action; in addition, ecotoxicological models are used for the detection, control and monitoring of the presence of pollutants in the environment. As a single bioassay will never provide a full picture of the quality of the environment, a representative, cost-effective and quantitative test battery should be developed. The effects of pentachlorophenol were studied using a battery of ecotoxicological model systems, including immobilization of Daphnia magna, bioluminiscence inhibition in the bacterium Vibrio fischeri, growth inhibition of the alga Chlorella vulgaris, and micronuclei induction in the plant Allium cepa. The inhibition of cell proliferation and MTT reduction were investigated in Vero cells. Neutral red uptake, cell growth, MTT reduction, lactate dehydrogenase leakage and activity were studied in the salmonid fish cell line RTG-2, derived from the gonad of rainbow trout. Pentachlorophenol was very toxic for all biota and cells. The system most sensitive to pentachlorophenol, was micronuclei induction in A. cepa, followed by D. magna immobilization, bioluminescence inhibition in V. fischeri bacteria at 60 min and cell proliferation inhibition of RTG-2 cells at 72 h. Inhibition of cell proliferation and MTT reduction on Vero monkey cells showed intermediate sensitivity.
G Repetto; A Jos; M J Hazen; M L Molero; A del Peso; M Salguero; P D Castillo; M C Rodríguez-Vicente; M Repetto
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  15     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:    2001 Aug-Oct
Date Detail:
Created Date:  2001-09-21     Completed Date:  2001-12-04     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
National Institute of Toxicology, PO Box 863, 41080 Seville, Spain.
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MeSH Terms
Cell Division / drug effects
Cercopithecus aethiops
Chlorella / drug effects,  growth & development
Daphnia / drug effects,  physiology
Dose-Response Relationship, Drug
Environmental Pollutants / toxicity*
L-Lactate Dehydrogenase / metabolism
Luminescent Measurements
Micronuclei, Chromosome-Defective / drug effects
Models, Biological
Neutral Red / metabolism
Oncorhynchus mykiss
Onions / drug effects,  genetics
Pentachlorophenol / toxicity*
Tetrazolium Salts / metabolism
Toxicity Tests / methods*
Vero Cells
Vibrio / drug effects,  physiology
Reg. No./Substance:
0/Environmental Pollutants; 0/Tetrazolium Salts; 553-24-2/Neutral Red; 87-86-5/Pentachlorophenol; EC Dehydrogenase

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