Document Detail


A temporally regulated, diffusible activity is required for rod photoreceptor development in vitro.
MedLine Citation:
PMID:  1618156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The retina is a relatively simple and well-characterized CNS structure in which cell-cell interactions have been hypothesized to influence cell type determination. By manipulating cell density in serum-free cultures we show that rat rod photoreceptor development requires a diffusible activity produced by neonatal retinal cells. This effect is not mediated by changes in cell survival or mitosis. Production of the rod promoting activity varies with developmental stage and is temporally correlated with the timing of rod generation in vivo. In low density cultures, which do not support rod development, an increased fraction of cells stain with an antibody specific for another retinal neuron, the bipolar cell. Thus, the diffusible rod promoting activity may influence cell fate determination, and not only terminal differentiation. These results provide an approach for the molecular characterization of developmentally important signals in the vertebrate retina.
Authors:
D Altshuler; C Cepko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  114     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-07-31     Completed Date:  1992-07-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  947-57     Citation Subset:  IM    
Affiliation:
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / physiology
Cells, Cultured
Immunohistochemistry
Microscopy, Fluorescence
Photoreceptor Cells / embryology*
Rats
Rats, Inbred Strains
Retina / cytology,  embryology,  physiology
Grant Support
ID/Acronym/Agency:
EY08864/EY/NEI NIH HHS; T32 EY07110/EY/NEI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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