Document Detail

A temporal model of cofilin regulation and the early peak of actin barbed ends in invasive tumor cells.
MedLine Citation:
PMID:  21504724     Owner:  NLM     Status:  MEDLINE    
Cofilin is an important regulator of actin polymerization, cell migration, and chemotaxis. Recent experimental data on mammary carcinoma cells reveal that stimulation by epidermal growth factor (EGF) generates a pool of active cofilin that results in a peak of actin filament barbed ends on the timescale of 1 min. Here, we present results of a mathematical model for the dynamics of cofilin and its transition between several pools in response to EGF stimulation. We describe the interactions of phospholipase C, membrane lipids (PIP(2)), and cofilin bound to PIP(2) and to F-actin, as well as diffusible cofilin in active G-actin-monomer-bound or phosphorylated states. We consider a simplified representation in which the thin cell edge (lamellipod) and the cell interior are represented by two compartments that are linked by diffusion. We demonstrate that a high basal level of active cofilin stored by binding to PIP(2), as well as the highly enriched local milieu of F-actin at the cell edge, is essential to capture the EGF-induced barbed-end amplification observed experimentally.
Nessy Tania; Erin Prosk; John Condeelis; Leah Edelstein-Keshet
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  100     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-20     Completed Date:  2011-08-11     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1883-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Actin Depolymerizing Factors / metabolism*
Actins / chemistry,  metabolism*
Breast Neoplasms / pathology*
Lim Kinases / metabolism
Models, Biological*
Neoplasm Invasiveness
Grant Support
Reg. No./Substance:
0/Actin Depolymerizing Factors; 0/Actins; EC Kinases

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