Document Detail

A technique to study the relationship between adipose cell size and lipogenesis in a heterogeneous population of adipose cells.
MedLine Citation:
PMID:  7462808     Owner:  NLM     Status:  MEDLINE    
A technique is described for the calculation of the incorporation of radioactive substrate into lipid in adipose cells that have been isolated and separated into groups according to their diameter from a single sample of adipose tissue containing a heterogeneous population of cells. After incorporation of radioactive substrate, a section of adipose tissue is fixed in osmium tetroxide and the fixed cells isolated and separated into specific diameter ranges using a series of nylon screens. The separated cells are weighed, decolorized with hydrogen peroxide, and the radioactivity is determined in the cells from each diameter range. With this method, true comparisons can be made between adipose cell size and lipogenic activity of isolated cells of known diameter which have been subjected to various nutritional or hormonal treatments. Results with sheep adipose tissue show that large adipose cells are considerably more active in the synthesis of lipid than small cells from the same sample of adipose tissue.
R L Hood; R F Thornton
Related Documents :
19203788 - The engineering of patient-specific, anatomically shaped, digits.
20966538 - A composite swnt-collagen matrix: characterization and preliminary assessment as a cond...
17319938 - Intrinsic differentiation potential of adolescent human tendon tissue: an in-vitro cell...
20132738 - Inhibition of the expression on mmp-2, 9 and morphological changes via human fibrosarco...
16380438 - Pax3 and pax7 have distinct and overlapping functions in adult muscle progenitor cells.
15988058 - Adenovirus-mediated overexpression of murine cyclic nucleotide phosphodiesterase 3b.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  21     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1980 Nov 
Date Detail:
Created Date:  1981-04-13     Completed Date:  1981-04-13     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1132-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acetates / metabolism
Adipose Tissue / cytology*,  metabolism
Cell Separation / instrumentation*
Lipids / biosynthesis*
Reg. No./Substance:
0/Acetates; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Abnormalities in lipoproteins of d < 1.006 g/ml in familial lecithin:cholesterol acyltransferase def...
Next Document:  Enhancement of the Yu and Ledeen gas-liquid chromatographic method for sialic acid estimation: use o...