Document Detail

A technique to study the relationship between adipose cell size and lipogenesis in a heterogeneous population of adipose cells.
MedLine Citation:
PMID:  7462808     Owner:  NLM     Status:  MEDLINE    
A technique is described for the calculation of the incorporation of radioactive substrate into lipid in adipose cells that have been isolated and separated into groups according to their diameter from a single sample of adipose tissue containing a heterogeneous population of cells. After incorporation of radioactive substrate, a section of adipose tissue is fixed in osmium tetroxide and the fixed cells isolated and separated into specific diameter ranges using a series of nylon screens. The separated cells are weighed, decolorized with hydrogen peroxide, and the radioactivity is determined in the cells from each diameter range. With this method, true comparisons can be made between adipose cell size and lipogenic activity of isolated cells of known diameter which have been subjected to various nutritional or hormonal treatments. Results with sheep adipose tissue show that large adipose cells are considerably more active in the synthesis of lipid than small cells from the same sample of adipose tissue.
R L Hood; R F Thornton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  21     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1980 Nov 
Date Detail:
Created Date:  1981-04-13     Completed Date:  1981-04-13     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1132-6     Citation Subset:  IM    
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MeSH Terms
Acetates / metabolism
Adipose Tissue / cytology*,  metabolism
Cell Separation / instrumentation*
Lipids / biosynthesis*
Reg. No./Substance:
0/Acetates; 0/Lipids

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