| A technique for the perfusion of the isolated rabbit pancreas. | |
| | |
MedLine Citation:
|
PMID: 6716717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The present paper describes a technique for the vascular perfusion of the isolated rabbit pancreas, evidence of the viability of the perfused gland and the responses to stimulation by secretin (Sc) and acetylcholine (ACh). The isolated rabbit pancreas was perfused at a constant pressure through the superior mesenteric artery with Krebs-Henseleit solution. The perfused gland, kept in a viable state over the perfusion period of 7 hr, secreted pancreatic juice spontaneously; the juice flow increased and the amylase output decreased gradually for approx. 2 hr after the beginning of perfusion, following the flow and output relatively constantly for several hours. The single infusion of Sc caused a prolonged flow of the pancreatic juice with an increase in the bicarbonate concentration and a decrease in the chloride concentration, though Sc was present in the perfusate for only a short time. The single infusion of ACh was not effective on the juice flow, but immediately increased the amylase output dose-dependently. These results indicate that the saline-perfused preparation of the rabbit pancreas has wide applications in experimental studies on the exocrine pancreas. |
| | |
Authors:
|
T Saito |
Related Documents
:
|
9006547 - Characteristics of thoracic duct lymph in multiple organ dysfunction syndrome. 12145167 - Islet hyperperfusion during prediabetic phase in oletf rats, a model of type 2 diabetes. 6698077 - Cardiovascular and lymph flow changes caused by physiological hyperosmolar provocation ... 1723097 - New method for the determination of pancreatic amylase evaluated. 4046867 - Microvessel diameter changes during hemorrhagic shock in unanesthetized hamsters. 15226957 - In vivo recording of blood velocity profiles and studies in vitro of profile alteration... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Japanese journal of pharmacology Volume: 34 ISSN: 0021-5198 ISO Abbreviation: Jpn. J. Pharmacol. Publication Date: 1984 Jan |
Date Detail:
|
Created Date: 1984-06-06 Completed Date: 1984-06-06 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2983305R Medline TA: Jpn J Pharmacol Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 43-50 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylcholine
/
administration & dosage Animals Female Male Pancreas* / secretion Pancreatic Juice / secretion Perfusion / methods* Rabbits Secretin / administration & dosage |
| Chemical | |
Reg. No./Substance:
|
1393-25-5/Secretin; 51-84-3/Acetylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Studies on antinephritic effect of mizoribine (p-INN, Bredinin), a new immunosuppressive agent, and ...
Next Document: The effects of Ca antagonists, manganese and lanthanum on cooling-induced contracture of depolarized...