Document Detail

A technique for demonstrating the nerve supply of whole larynges.
MedLine Citation:
PMID:  1379438     Owner:  NLM     Status:  MEDLINE    
At present there is a large gap in our understanding of the exact distribution of sensory and motor nerves within the larynx. Gross dissection is only accurate for large-caliber nerves, whereas microscopic sections show only isolated examples of terminal branching. To fill this gap, we modified an old technique called Sihler's stain to process five whole canine larynges. The technique requires eight steps that take approximately 3 months. The result is an entire larynx in which muscle and cartilage are rendered translucent, while nerve is counterstained. Nerve branching can be continuously traced from the large-caliber nerves to the terminal branches. We found that the nerve patterns are surprisingly complex and contain frequent anastomotic networks. This technique is a powerful tool for studying certain neurolaryngological problems.
B L Wu; I Sanders
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of otolaryngology--head & neck surgery     Volume:  118     ISSN:  0886-4470     ISO Abbreviation:  Arch. Otolaryngol. Head Neck Surg.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-09-08     Completed Date:  1992-09-08     Revised Date:  2006-03-28    
Medline Journal Info:
Nlm Unique ID:  8603209     Medline TA:  Arch Otolaryngol Head Neck Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  822-7     Citation Subset:  AIM; IM    
Grabscheid Voice Center, Department of Otolaryngology, Mount Sinai Medical Center, New York, NY 10029-6574.
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MeSH Terms
Histological Techniques
Laryngeal Nerves / anatomy & histology*
Recurrent Laryngeal Nerve / anatomy & histology
Staining and Labeling / methods*

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