Document Detail


A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells.
MedLine Citation:
PMID:  18605728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present the design, synthesis, and biological applications of mitochondria peroxy yellow 1 (MitoPY1), a new type of bifunctional fluorescent probe for imaging hydrogen peroxide levels within the mitochondria of living cells. MitoPY1 combines a chemoselective boronate-based switch and a mitochondrial-targeting phosphonium moiety for detection of hydrogen peroxide localized to cellular mitochondria. Confocal microscopy and flow cytometry experiments in a variety of mammalian cell types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide concentrations generated by situations of oxidative stress.
Authors:
Bryan C Dickinson; Christopher J Chang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-07-08
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  130     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-24     Completed Date:  2008-08-26     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9638-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
COS Cells
Cercopithecus aethiops
Cricetinae
Cricetulus
Fluorescent Dyes / chemical synthesis,  chemistry*
HeLa Cells
Humans
Hydrogen Peroxide / analysis*,  metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Mitochondria / chemistry*,  metabolism
Spectrometry, Fluorescence
Grant Support
ID/Acronym/Agency:
GM 79465/GM/NIGMS NIH HHS; R01 GM079465/GM/NIGMS NIH HHS; R01 GM079465-03/GM/NIGMS NIH HHS; T32 GM066698/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; BBX060AN9V/Hydrogen Peroxide
Comments/Corrections
Erratum In:
J Am Chem Soc. 2008 Aug 27;130(34):11561

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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