| A target of thrombin activation promotes cell cycle re-entry by urodele muscle cells. | |
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MedLine Citation:
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PMID: 9824556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A key early event of newt limb regeneration is the local dedifferentiation of cells to form dividing progenitor cells. This involves the plasticity of differentiation and the ability to re-enter the cell cycle. In culture, differentiated newt myotubes are able to re-enter S-phase in response to serum stimulation. Here, we analyzed the intracellular and extracellular requirements for this process. Cell cycle re-entry depends on the phosphorylation of the retinoblastoma protein, which is a key regulator of the G1-S transition. This is in contrast to mammalian myotubes, which are refractory to serum stimulation and cannot phosphorylate retinoblastoma protein in response to serum. The serum factor responsible for this phosphorylation appears to be distinct from common polypeptide growth factors and is enriched in crude preparations of bovine thrombin. Fractionation and analysis of this preparation indicate that the factor is regulated by thrombin and plasmin proteolysis. These results indicate that factors involved in acute responses to wounding such as clotting may be important initiators of the regenerative response. |
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Authors:
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E M Tanaka; J P Brockes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society Volume: 6 ISSN: 1067-1927 ISO Abbreviation: Wound Repair Regen Publication Date: 1998 Jul-Aug |
Date Detail:
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Created Date: 1999-01-14 Completed Date: 1999-01-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9310939 Medline TA: Wound Repair Regen Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 371-81 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University College, London; and the Ludwig Institute for Cancer Research, Middlesex Hospital Branch, London, England. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Cycle / physiology* Cells, Cultured Chromatography Culture Media DNA / biosynthesis Forelimb / physiology* Muscles / cytology*, metabolism Phosphorylation Regeneration / physiology* Thrombin / analysis, metabolism* Urodela |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 9007-49-2/DNA; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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