Document Detail


A tandemly repeated DNA sequence is associated with both knob-like heterochromatin and a highly decondensed structure in the meiotic pachytene chromosomes of rice.
MedLine Citation:
PMID:  11398973     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Highly repetitive tandem DNA sequence repeats are often associated with centromeric and telomeric regions of eukaryotic chromosomes. The rice tandem repeat Os48 is organized as long arrays of a 355 bp monomer and is mainly located in the telomeric regions. The chromosomal locations of the Os48 sequence were determined by fluorescence in situ hybridization (FISH) on rice pachytene chromosomes. The majority of the Os48 loci are associated with brightly 4',6-diamidino-2-phenylindole (DAPI)-stained and knob-like heterochromatin in rice pachytene chromosomes. As with other DNA sequences located in the heterochromatic regions, the cytosines of the CG and C(A/T)G sites within the Os48 repeat are heavily methylated. Surprisingly, a proportion of the FISH signals are highly decondensed and deviate significantly from the DAPI-stained periphery of the pachytene chromosomes. This highly decondensed chromatin structure has not been reported in pachytene chromosomes prepared from alcohol/acid-fixed meiotic samples in any other eukaryotic species. The condensation of the Os48 sequences is dynamic during prophase I of meiosis. The FISH signals derived from the Os48 repeat progress from a condensed configuration between leptonema and early pachynema into a decondensed structure from middle pachynema to diakinesis, and then return to a condensed form at metaphase I.
Authors:
Z Cheng; R M Stupar; M Gu; J Jiang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosoma     Volume:  110     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-06-11     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  24-31     Citation Subset:  IM    
Affiliation:
Department of Horticulture, University of Wisconsin-Madison, 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes / ultrastructure
DNA Methylation
DNA, Plant / genetics*
Genome, Plant
Heterochromatin / genetics,  ultrastructure
In Situ Hybridization, Fluorescence
Meiosis
Oryza sativa / genetics*,  ultrastructure
Physical Chromosome Mapping
Tandem Repeat Sequences
Transcription, Genetic
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Heterochromatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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