| tPA of human keratinocytes: contribution to cell surface-associated plasminogen activation and upregulation by retinoic acid. | |
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MedLine Citation:
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PMID: 8608343 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We tested distinct variants of a human keratinocyte line (HaCaT) for the expression of tissue-type plasminogen activator (tPA)-specific mRNA, as well as cell surface-associated and secreted tPA. Cells of early passages (passage no. 22) only expressed urokinase plasminogen activator (uPA)- but not tPA-specific mRNA. Cells after prolonged culture (passage no. 44) expressed uPA- and tPA-specific mRNA, but did not release tPA in the extracellular space and did not display surface-associated tPA. HaCaT cells transformed with the c-Ha-ras oncogene (HaCaTras) showed both secreted and surface-associated tPA antigen. The secreted and the surface-associated plasminogen activator (PA)-activity of HaCaTras cells were in part inhibitable by anticatalytic anti-tPA antibodies, thus indicating that tPA contributes to extracellular and surface-associated plasminogen activation. Finally, we demonstrate that tPA secretion of HaCaT 44 cells can be induced by retinoic acid, most likely via interaction of retinoic acid with nuclear-associated retinoic acid-receptor(s). |
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Authors:
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F Buessecker; J Reinartz; M D Kramer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental dermatology Volume: 4 ISSN: 0906-6705 ISO Abbreviation: Exp. Dermatol. Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1996-05-24 Completed Date: 1996-05-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 357-64 Citation Subset: IM |
Affiliation:
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Institut für Immunologie der Universität Laboratorium für Immunpathologie, Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Line Enzyme-Linked Immunosorbent Assay Humans Keratinocytes / cytology, drug effects, metabolism* Keratolytic Agents / pharmacology* Molecular Sequence Data RNA, Messenger / drug effects, metabolism Tissue Plasminogen Activator / drug effects, genetics, metabolism* Tretinoin / pharmacology* Up-Regulation Urokinase-Type Plasminogen Activator / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Keratolytic Agents; 0/RNA, Messenger; 302-79-4/Tretinoin; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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