Document Detail


A systems biology approach sheds new light on Escherichia coli acid resistance.
MedLine Citation:
PMID:  21690099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to develop an infection, diarrhogenic Escherichia coli has to pass through the stomach, where the pH can be as low as 1. Mechanisms that enable E. coli to survive in low pH are thus potentially relevant for pathogenicity. Four acid response systems involved in reducing the concentration of intracellular protons have been identified so far. However, it is still unclear to what extent the regulation of other important cellular functions may be required for survival in acid conditions. Here, we have combined molecular and phenotypic analysis of wild-type and mutant strains with computational network inference to identify molecular pathways underlying E. coli response to mild and strong acid conditions. The interpretative model we have developed led to the hypothesis that a complex transcriptional programme, dependent on the two-component system regulator OmpR and involving a switch between aerobic and anaerobic metabolism, may be key for survival. Experimental validation has shown that the OmpR is responsible for controlling a sizeable component of the transcriptional programme to acid exposure. Moreover, we found that a ΔompR strain was unable to mount any transcriptional response to acid exposure and had one of the strongest acid sensitive phenotype observed.
Authors:
Anna Stincone; Nazish Daudi; Ayesha S Rahman; Philipp Antczak; Ian Henderson; Jeffrey Cole; Matthew D Johnson; Peter Lund; Francesco Falciani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-19
Journal Detail:
Title:  Nucleic acids research     Volume:  39     ISSN:  1362-4962     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-22     Completed Date:  2011-12-09     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  7512-28     Citation Subset:  IM    
Affiliation:
The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / genetics
Bacterial Proteins / genetics,  physiology
Cell Wall / metabolism
Energy Metabolism
Escherichia coli / genetics*,  metabolism
Gene Expression Regulation, Bacterial*
Gene Regulatory Networks*
Hydrogen-Ion Concentration
Mutation
Phenotype
Systems Biology
Trans-Activators / genetics,  physiology
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
BBC5151041//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Trans-Activators; 0/osmolarity response regulator proteins
Comments/Corrections

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