Document Detail

A systematic approach towards the complete assignment of 13C resonances for horse ferrocytochrome c.
MedLine Citation:
PMID:  2176594     Owner:  NLM     Status:  MEDLINE    
The complete 1H-NMR assignments for horse ferrocytochrome c have been reported by Wand and colleagues and by our group at Oxford. Using these 1H assignments, we now report chemical shift assignment for 205 13C resonances arising from horse ferrocytochrome c. This is from a total of 437 13C nuclei with covalently attached protons. These chemical shift assignments have been achieved using 1H-detected two-dimensional heteronuclear 1H-13C correlation techniques. The data have been collected from samples of horse ferrocytochrome c without isotopic enrichment. The complete 13C assignments for all carbons with covalently linked protons are reported for the amino acids Ala, Thr, Val and Gly. Specific assignments are tabulated for all 49 methyl groups, for 52 of 92 alpha-carbon resonances, for 10 resonances associated with the heme group, for all aromatic side-chain 13C resonances which have covalent protons and give rise to observable cross peaks under the experimental conditions used, as well as for a number of other side chains of aliphatic amino acids.
Y Gao; J Boyd; R J Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  194     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-02-20     Completed Date:  1991-02-20     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  355-65     Citation Subset:  IM    
Inorganic Chemistry Laboratory, University of Oxford, UK.
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MeSH Terms
Amino Acids / chemistry
Carbon Isotopes
Cytochrome c Group / chemistry*
Magnetic Resonance Spectroscopy
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Cytochrome c Group

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