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A system for incubations at high gas partial pressure.
MedLine Citation:
PMID:  22347218     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
High-pressure is a key feature of deep subsurface environments. High partial pressure of dissolved gasses plays an important role in microbial metabolism, because thermodynamic feasibility of many reactions depends on the concentration of reactants. For gases, this is controlled by their partial pressure, which can exceed 1 MPa at in situ conditions. Therefore, high hydrostatic pressure alone is not sufficient to recreate true deep subsurface in situ conditions, but the partial pressure of dissolved gasses has to be controlled as well. We developed an incubation system that allows for incubations at hydrostatic pressure up to 60 MPa, temperatures up to 120°C, and at high gas partial pressure. The composition and partial pressure of gasses can be manipulated during the experiment. To keep costs low, the system is mainly made from off-the-shelf components with only very few custom-made parts. A flexible and inert PVDF (polyvinylidene fluoride) incubator sleeve, which is almost impermeable for gases, holds the sample and separates it from the pressure fluid. The flexibility of the incubator sleeve allows for sub-sampling of the medium without loss of pressure. Experiments can be run in both static and flow-through mode. The incubation system described here is usable for versatile purposes, not only the incubation of microorganisms and determination of growth rates, but also for chemical degradation or extraction experiments under high gas saturation, e.g., fluid-gas-rock-interactions in relation to carbon dioxide sequestration. As an application of the system we extracted organic compounds from sub-bituminous coal using H(2)O as well as a H(2)O-CO(2) mixture at elevated temperature (90°C) and pressure (5 MPa). Subsamples were taken at different time points during the incubation and analyzed by ion chromatography. Furthermore we demonstrated the applicability of the system for studies of microbial activity, using samples from the Isis mud volcano. We could detect an increase in sulfate reduction rate upon the addition of methane to the sample.
Authors:
Patrick Sauer; Clemens Glombitza; Jens Kallmeyer
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Publication Detail:
Type:  Journal Article     Date:  2012-02-03
Journal Detail:
Title:  Frontiers in microbiology     Volume:  3     ISSN:  1664-302X     ISO Abbreviation:  Front Microbiol     Publication Date:  2012  
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-10-02     Revised Date:  2013-08-13    
Medline Journal Info:
Nlm Unique ID:  101548977     Medline TA:  Front Microbiol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  25     Citation Subset:  -    
Affiliation:
Geomicrobiology Group, Institute for Earth and Environmental Sciences, University of Potsdam Potsdam, Germany.
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