Document Detail


The synthetic kappa-opioid agonist (-)U50,488 does not affect calcium transport into R1.1 mouse thymoma cell line.
MedLine Citation:
PMID:  10230876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, the effect of the synthetic kappa-opioid agonist (-)U50,488 on 45Ca2- transport into R1.1 mouse thymoma cells is presented. This thymoma cell line expresses selectively the kappa-opioid class of receptors. 45Ca2+ transport into R1.1 cells was not affected by the kappa-opioid agonist (-)U50,488 (10(-10) M-10(-4) M) alone, or in the presence of the plant lectins: PHA (250 microg/ml) and Con A (800 microg/ml), after a 60 min treatment. The plant lectins PHA and Con A stimulated 45Ca2+ transport into R1.1 cells, in high concentrations (100-800 microg/ml) and (200-1000 microg/ml) respectively, after a 60 min treatment. Thus, 45Ca2+ transport was not affected in R1.1 cells by the kappa-opioid agonist (-)U50,488 alone, or in the presence of mitogens after a 60 min treatment. This negative result does not indicate the lack of calcium channels on R1.1 cells, since the plant lectins PHA and Con A were able to stimulate 45Ca2+ transport into R1.1 cells.
Authors:
I Martin-Kleiner; J M Bidlack
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of immunopharmacology     Volume:  21     ISSN:  0192-0561     ISO Abbreviation:  Int. J. Immunopharmacol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-10     Completed Date:  1999-06-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7904799     Medline TA:  Int J Immunopharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Affiliation:
Ruder Bosković Institute, Division of Molecular Medicine, Zagreb, Croatia.
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MeSH Terms
Descriptor/Qualifier:
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / pharmacology*
Animals
Calcium / metabolism*
Concanavalin A / pharmacology
Ion Transport / drug effects
Mice
Phytohemagglutinins / pharmacology
Receptors, Opioid, kappa / agonists*
Thymoma / metabolism*
Thymus Neoplasms / metabolism*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
DA 04355/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Phytohemagglutinins; 0/Receptors, Opioid, kappa; 11028-71-0/Concanavalin A; 67198-13-4/3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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