Document Detail

The synthesis of ribonucleic acid in vivo in the nuclei of rat brain fractionated by zonal centrifugation.
MedLine Citation:
PMID:  4721614     Owner:  NLM     Status:  MEDLINE    
1. Radioactive orotic acid, uridine and adenosine were administered to rats by intracisternal injection. The effects of the size of the dose, the specific radioactivity and time on the incorporation into the RNA of unfractionated nuclei of brain tissue were examined to establish appropriate conditions for studies of the relative activities in vivo of the various sorts of brain nuclei fractionated by zonal centrifugation. Uridine is incorporated more efficiently than either orotic acid or adenosine. 2. With [(3)H]uridine as precursor the astrocytes in zone (II) contain the highest radioactivity except at the beginning of the experiment when the neuronal nuclei of zone (I) are more highly labelled. This fraction utilizes [(14)C]orotic acid more readily than the nuclei of zone (II). The astrocytic nuclei of zone (III) show a general resemblance to those of zone (II). Considerable differences between the incorporations into the two types of oligodendrocyte nuclei in zones (IV) and (V) are observed. 3. The relative synthetic activities of the major types of brain nuclei in vitro and in vivo are discussed.
J Austoker; D Cox; A P Mathias
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  132     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 Apr 
Date Detail:
Created Date:  1973-10-05     Completed Date:  1973-10-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  813-9     Citation Subset:  IM    
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MeSH Terms
Adenosine / metabolism
Brain / cytology,  metabolism*
Carbon Isotopes
Cell Fractionation
Cell Nucleus / metabolism*
Centrifugation, Zonal
Orotic Acid / metabolism
RNA / biosynthesis*
Time Factors
Uridine / metabolism
Reg. No./Substance:
0/Carbon Isotopes; 10028-17-8/Tritium; 58-61-7/Adenosine; 58-96-8/Uridine; 63231-63-0/RNA; 65-86-1/Orotic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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