Document Detail


The synthesis and properties of oligoribonucleotide-spermine conjugates.
MedLine Citation:
PMID:  15254639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyamines stabilise nucleic acids against chemical and enzymatic degradation, facilitate the formation of secondary and tertiary structures and enhance cellular uptake. Therefore methods for the syntheses of polyamine-nucleic acid conjugates are of interest. A route for the syntheses of RNA-spermine conjugates has been developed. The polyamine was introduced to the C-5 position of uridine via an ethyl tether and the molecule elaborated into a synthon suitable for oligoribonucleotide assembly. The resultant oligomers were components of the hairpin ribozyme. Characterisation of the spermine-conjugated catalytic RNA revealed that attachment of the polyamine was well tolerated in three of four positions, namely U41, U37 and U34, suggesting that conjugation to C-5 brings about minimal structural perturbation.
Authors:
Andrew J Marsh; David M Williams; Jane A Grasby
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-06-28
Journal Detail:
Title:  Organic & biomolecular chemistry     Volume:  2     ISSN:  1477-0520     ISO Abbreviation:  Org. Biomol. Chem.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-15     Completed Date:  2004-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101154995     Medline TA:  Org Biomol Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  2103-12     Citation Subset:  IM    
Affiliation:
Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Brook Hill, Sheffield, UKS3 7HF.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Molecular Sequence Data
Molecular Structure
Oligoribonucleotides / chemical synthesis*,  chemistry*
Polyamines / chemistry
RNA / chemical synthesis
RNA, Catalytic / chemical synthesis,  chemistry
Spermine / chemical synthesis*,  chemistry*
Uridine / chemical synthesis,  chemistry
Chemical
Reg. No./Substance:
0/Oligoribonucleotides; 0/Polyamines; 0/RNA, Catalytic; 0/hairpin ribozyme; 58-96-8/Uridine; 63231-63-0/RNA; 71-44-3/Spermine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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