Document Detail

The synthesis of phosphatidylcholine by adult rat lung alveolar type II epithelial cells in primary culture.
MedLine Citation:
PMID:  565218     Owner:  NLM     Status:  MEDLINE    
1. The formation of phosphatidylcholine from radioactive precursors was studied in adult rat lung alveolar type II epithelial cells in primary culture. 2. The incorporation of [Me-14C]choline into total lipids and phosphatidylcholine was stimulated by addition of palmitate, whereas the incorporation of [U-14C]glucose into phosphatidylcholine and disaturated phosphatidylcholine was stimulated by addition of choline. Addition of glucose decreased the absolute rate of incorporation of [1(3)-3H]glycerol into total lipids, phosphatidylcholine and disaturated phosphatidylcholine, decreased the percentage [1(3)-3H]glycerol recovered in phosphatidylcholine, but increased the percentage phosphatidylcholine label in the disaturated species. 3. At saturating substrate concentrations, the percentages of phosphatidylcholine radioactivity found in disaturated phosphatidylcholine after incubation with [1-(14)C]acetate (in the presence of glucose) [1-(14)C]palmitate (in the presence of glucose), [Me-14C]choline (in the presence of glucose and palmitate) and [U-14C]glucose (in the presence of choline and palmitate) were 78, 75, 74 and 90%, respectively. 4. Fatty acids stimulated the incorporation of [U-14C]glucose into the glycerol moiety of phosphatidylcholine. The degree of unsaturation of the added fatty acids was reflected in the distribution of [U-14C]glucose label among the different molecular species of phosphatidylcholine. It is suggested that the glucose concentration in the blood as related to the amount of available fatty acids and their degree of unsaturation may be factors governing the synthesis of surfactant lipids.
J J Batenburg; W J Longmore; L M van Golde
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  529     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1978 Apr 
Date Detail:
Created Date:  1978-06-28     Completed Date:  1978-06-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  160-70     Citation Subset:  IM    
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MeSH Terms
Acetates / metabolism
Blood Glucose / metabolism
Cells, Cultured
Choline / metabolism
Epithelium / metabolism
Glucose / metabolism
Glycerol / metabolism
Palmitates / metabolism
Phosphatidylcholines / biosynthesis*
Pulmonary Alveoli / cytology,  metabolism*
Reg. No./Substance:
0/Acetates; 0/Blood Glucose; 0/Palmitates; 0/Phosphatidylcholines; 50-99-7/Glucose; 56-81-5/Glycerol; 62-49-7/Choline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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