Document Detail


The symbiotic ion channel homolog DMI1 is localized in the nuclear membrane of Medicago truncatula roots.
MedLine Citation:
PMID:  17173544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Legumes utilize a common signaling pathway to form symbiotic associations both with rhizobial bacteria and arbuscular mycorrhizal fungi. The perception of microbial signals is believed to take place at the plasma membrane, activating a cascade that converges on the nucleus where transcriptional reprogramming facilitates the symbioses. Forward genetic strategies have identified genes in this signaling pathway including Medicago truncatula DMI1 (Doesn't Make Infections 1) that encodes a putative ion channel. Although the DMI1 homologs from Lotus japonicus, CASTOR and POLLUX, were recently reported to be localized in plastids, we report here that a functional DMI1::GFP fusion is localized to the nuclear envelope in M. truncatula roots when expressed both from a constitutive 35S promoter and from a native DMI1 promoter. Localization may be mediated in part by sequences located within the amino-terminus of DMI1. This region of DMI1 is required for symbiotic signal transduction, and its replacement with a bona fide plastid transit peptide from the glutamine synthetase 2 gene does not restore DMI1 function. These new data place DMI1 in the nuclear envelope in close proximity to the origin of Nod-factor-induced calcium spiking.
Authors:
Brendan K Riely; Géraldine Lougnon; Jean-Michel Ané; Douglas R Cook
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-12-14
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  49     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-23     Completed Date:  2007-07-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  208-16     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / metabolism
Gene Expression Regulation, Plant
Green Fluorescent Proteins / genetics,  metabolism
Ion Channels / genetics,  metabolism*
Medicago truncatula / genetics,  metabolism*,  microbiology
Microscopy, Confocal
Nuclear Envelope / metabolism*
Plant Proteins / genetics,  metabolism*
Plant Roots / cytology,  genetics,  metabolism*,  microbiology
Plants, Genetically Modified
Promoter Regions, Genetic / genetics
Recombinant Fusion Proteins / genetics,  metabolism
Rhizobium / growth & development
Symbiosis
Chemical
Reg. No./Substance:
0/Ion Channels; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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