| The survey of porcine teschoviruses in field samples in China with a universal rapid probe real-time RT-PCR assay. | |
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MedLine Citation:
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PMID: 23138413 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A real-time reverse transcription polymerase chain reaction (RT-PCR) based on TaqMan was established and evaluated for quantitative detection of porcine teschoviruses (PTVs). A pair of primers and a TaqMan probe targeting on the highly conserved sequence of the 5'-untranslated region (5'-UTR) of one to 11 serotypes of PTV were designed. Standard plasmid DNA containing PCR amplification of the 5'-UTR were constructed and used to develop the real-time RT-PCR. The results indicated that the real-time RT-PCR was specific for detection of PTV with a detection limit of 10 copies/μL, but not for porcine parvovirus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, pseudorabies virus, classical swine fever virus. The coefficient of variation of inter-assay and intra-assay were less than 3 %. A total of 91 clinical samples were tested by the real-time RT-PCR and virus isolation (OIE 2008) and positive rates were 79.12 % (72/91) and 57.14 % (48/91), respectively. In conclusion, the developed real-time RT-PCR assay was an effective method for detection and quantification of PTV in fields or organs of infected pigs. |
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Authors:
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Chaofan Zhang; Zhongtian Wang; Feng Hu; Yebing Liu; Zheng Qiu; Shun Zhou; Shangjin Cui; Ming Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-9 |
Journal Detail:
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Title: Tropical animal health and production Volume: - ISSN: 1573-7438 ISO Abbreviation: Trop Anim Health Prod Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1277355 Medline TA: Trop Anim Health Prod Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Preventive, VeterinaryCollege of Veterinary Medicine, China Agricultural University, Beijing, 100094, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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