Document Detail


A survey on the cytogenetic status of the Croatian general population by use of the cytokinesis-block micronucleus assay.
MedLine Citation:
PMID:  18029222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytokinesis-block micronucleus assay (CBMN) was used to assess the variability and determine possible influences of external and internal factors on the background levels of cytogenetic damage in peripheral blood lymphocytes (PBL) of 50 healthy volunteers selected at random from the general population of Croatia. The mean MN frequency for all subjects was 4.74+/-0.31 per 1000 cells and the mean cytokinesis-block proliferation index (CBPI) was 1.82+/-0.01. The mean frequency of nucleoplasmic bridges (NPB) for all subjects was 0.06+/-0.04 and of nuclear buds (NB) 0.12+/-0.05. The canonical correlation analyses indicate a positive non-significant correlation between the MN frequency and age, gender and smoking habits. Results of factor structure and canonical weights showed that age and gender rather than smoking habits control the incidence of MN in PBL of healthy volunteers. The lowest median value of MN was observed in subjects younger than 30 years (both smoking and non-smoking). Generally, non-smokers had lower median values of MN compared to smokers. In non-smokers, males showed lower micronucleus incidence than females. Within the non-smokers smaller differences in the median values of MN between subgroups (male and female; age subgroups) were observed. Among smokers, females had a two-fold higher median value of MN frequency than males, but this difference was not significant (p=0.2643, Mann-Whitney U test). Canonical correlation analyses indicate a strong and significant correlation between cell proliferation parameters (M1-M4 and CBPI) and age, gender and smoking habits. The most sensitive parameters were M3 and M4. Age had the strongest effect on M3, while M4 was highly influenced by smoking habits. Gender had an equal non-significant effect on both parameters. The usefulness of the new criteria for the cytokinesis-block MN assay measuring DNA damage as a sensitive biomarker in biomonitoring studies is confirmed.
Authors:
Verica Garaj-Vrhovac; Martina Durinec; Nevenka Kopjar; Visnja Orescanin
Publication Detail:
Type:  Journal Article     Date:  2007-09-12
Journal Detail:
Title:  Mutation research     Volume:  649     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-05-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-100     Citation Subset:  IM    
Affiliation:
Institute for Medical Research and Occupational Health, Mutagenesis Unit, Zagreb, Croatia. vgaraj@imi.hr
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MeSH Terms
Descriptor/Qualifier:
Adult
Age Factors
Cell Proliferation*
Croatia
Cytokinesis / genetics*
DNA Damage
Female
Humans
Lymphocytes / cytology,  metabolism*
Male
Micronucleus Tests / methods
Middle Aged
Sex Factors
Smoking

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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