Document Detail


The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs.
MedLine Citation:
PMID:  9620853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nonsense-mediated mRNA decay pathway is an example of an evolutionarily conserved surveillance pathway that rids the cell of transcripts that contain nonsense mutations. The product of the UPF1 gene is a necessary component of the putative surveillance complex that recognizes and degrades aberrant mRNAs. Recent results indicate that the Upf1p also enhances translation termination at a nonsense codon. The results presented here demonstrate that the yeast and human forms of the Upf1p interact with both eukaryotic translation termination factors eRF1 and eRF3. Consistent with Upf1p interacting with the eRFs, the Upf1p is found in the prion-like aggregates that contain eRF1 and eRF3 observed in yeast [PSI+] strains. These results suggest that interaction of the Upf1p with the peptidyl release factors may be a key event in the assembly of the putative surveillance complex that enhances translation termination, monitors whether termination has occurred prematurely, and promotes degradation of aberrant transcripts.
Authors:
K Czaplinski; M J Ruiz-Echevarria; S V Paushkin; X Han; Y Weng; H A Perlick; H C Dietz; M D Ter-Avanesyan; S W Peltz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  12     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-01     Completed Date:  1998-07-01     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1665-77     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School-UMDNJ, USA.
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MeSH Terms
Descriptor/Qualifier:
Fungal Proteins / genetics*,  metabolism
Humans
Peptide Termination Factors / genetics*
Protein Biosynthesis*
RNA Helicases*
RNA, Messenger / genetics*,  metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Trans-Activators
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
GM48631-01/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/ETF1 protein, human; 0/Fungal Proteins; 0/Peptide Termination Factors; 0/RNA, Messenger; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; 0/UPF1 protein, human; 0/peptide-chain-release factor 3; EC 2.7.7.-/RNA Helicases; EC 3.6.1.-/NAM7 protein, S cerevisiae
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