| RA410/Sly1 suppresses MPP+ and 6-hydroxydopamine-induced cell death in SH-SY5Y cells. | |
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MedLine Citation:
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PMID: 15649705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Parkinson's disease is characterized by selective loss of dopaminergic neurons in the substantia nigra. However, its associated cell death mechanism remains unknown. 1-Methyl-4-phenil-pyridinium (MPP+) and 6-hydroxydopamine (6-OHDA) cause dopaminergic neuronal cell death. Both are widely used to model PD. We investigated the role of a vesicle-transport-related protein, RA410/Sly1, in SH-SY5Y cells to clarify the mechanism of cellular adaptation to MPP+ and 6-OHDA-induced stress. Antisense RA410/Sly1 transformants treated with these toxins displayed reduced viability in comparison with viability of wild-type or RA410/Sly1 sense transformants. Electron microscopy analysis indicated that the ER in MPP+-treated antisense RA410/Sly1 transformants was rapidly disrupted in comparison to wild-type or sense RNA transformants. Cell death induced by MPP+ and 6-OHDA was suppressed in RA410/Sly1 sense transformants through suppression of caspase-2, -3 and -9 activation. These results suggest that RA410/Sly1 plays an important cytoprotective role in MPP+ and 6-OHDA-induced cellular perturbation. |
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Authors:
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Yoshio Bando; Taiichi Katayama; Manabu Taniguchi; Tomohiko Ishibashi; Noriyuki Matsuo; Satoshi Ogawa; Masaya Tohyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurobiology of disease Volume: 18 ISSN: 0969-9961 ISO Abbreviation: Neurobiol. Dis. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-01-14 Completed Date: 2005-05-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9500169 Medline TA: Neurobiol Dis Country: United States |
Other Details:
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Languages: eng Pagination: 143-51 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan. ybando@asahikawa-med.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-4-phenylpyridinium
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toxicity Adaptation, Physiological / physiology Animals Apoptosis / drug effects, physiology* Carrier Proteins / antagonists & inhibitors, genetics, physiology* Caspases / metabolism Cell Survival / drug effects, physiology Dopamine / metabolism Endoplasmic Reticulum / drug effects, pathology, ultrastructure Humans Immediate-Early Proteins / antagonists & inhibitors, genetics, physiology* Microscopy, Electron, Transmission Munc18 Proteins Nerve Degeneration / chemically induced, metabolism*, pathology Neurons / drug effects, pathology, ultrastructure Neurotoxins / toxicity* Oligonucleotides, Antisense / pharmacology Oxidative Stress / drug effects, physiology* Oxidopamine / toxicity Parkinsonian Disorders / chemically induced, metabolism, pathology Rats Substantia Nigra / drug effects, pathology, physiopathology Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Immediate-Early Proteins; 0/Munc18 Proteins; 0/Neurotoxins; 0/Oligonucleotides, Antisense; 0/Scfd1 protein, rat; 1199-18-4/Oxidopamine; 48134-75-4/1-Methyl-4-phenylpyridinium; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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