Document Detail


A supplemented soft agar chemotaxis assay demonstrates the Helicobacter pylori chemotactic response to zinc and nickel.
MedLine Citation:
PMID:  23139399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Directed motility, or chemotaxis, is required for Helicobacter pylori to establish infection in the stomach, although the full repertoire of this bacterium's chemotactic responses is not yet known. Here we report that H. pylori responds to zinc as an attractant and nickel as a repellent. To reach this conclusion, we employed both a temporal chemotaxis assay based on bacterial reversals and a supplemented soft agar spatial assay. We refined the temporal assay using a previously described chemorepellent, acid, and found that H. pylori requires rich media with serum to maintain optimal swimming motility. Surprisingly, we found that some strains respond to acid as an attractant, and that the TlpC chemoreceptor correlated with whether acid was sensed as an attractant or repellent. Using this same assay, we detected weak repellent responses to nickel and copper, and a varied response to zinc. We thus developed an alternative spatial chemotactic assay called the supplemented soft agar assay, which utilizes soft agar medium supplemented with the test compound. With Escherichia coli, the attractant serine slowed overall bacterial migration, while the repellent nickel increased the speed of overall migration. In H. pylori we detected slowed migration with doubled tryptone media, as well as zinc, consistent with an attractant response. In contrast, nickel increased migration, consistent with repulsion.
Authors:
Lisa Sanders; Tessa M Andermann; Karen M Ottemann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-08
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  159     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-26     Completed Date:  2013-05-29     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  46-57     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism
Agar
Bacteriological Techniques / methods
Chemotaxis*
Copper / metabolism
Culture Media / chemistry
Escherichia coli / metabolism
Helicobacter pylori / drug effects,  metabolism,  physiology*
Nickel / metabolism*
Serine / metabolism
Zinc / metabolism*
Grant Support
ID/Acronym/Agency:
AI050000/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Acids; 0/Culture Media; 452VLY9402/Serine; 789U1901C5/Copper; 7OV03QG267/Nickel; 9002-18-0/Agar; J41CSQ7QDS/Zinc
Comments/Corrections

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