Document Detail

The superantigen toxic shock syndrome toxin-1 induces CD40 ligand expression and modulates IgE isotype switching.
MedLine Citation:
PMID:  8921429     Owner:  NLM     Status:  MEDLINE    
Isotype switching to IgE requires two signals. The first signal is provided by the cytokines IL-4 or IL-13, and the second signal is delivered by the interaction between the B cell antigen CD40 and its ligand (CD40L) which is expressed on activated T cells. Since superantigens have been shown to activate T cells, we examined the effect of the superantigen toxic shock syndrome toxin-1 (TSST-1) on CD40L expression on T cells. TSST-1 induced expression of CD40L in both freshly isolated T cells and in T cell lines expanded by re-stimulation with TSST-1. CD40L was preferentially expressed in the V beta 2 subset of T cells expanded by TSST-1. We next examined the effect of TSST-1 on IgE synthesis by human peripheral blood mononuclear cells (PBMC). Addition of TSST-1 to PBMC inhibited IL-4-induced IgE synthesis in a dose-dependent manner. This inhibition was reversed partly by adding a neutralizing antibody to IFN-gamma. In contrast, TSST-1 induced high amounts of IgE synthesis in the presence of IL-4 at low T:B cell ratios (0.5:10 to 4:10), a condition which circumvents the inhibitory effect of IFN-gamma. TSST-1 induction of IgE synthesis was inhibited by a mAb to CD40L. These results indicate that superantigens induce CD40L expression in T cells and cause isotype switching in B cells which is mediated by CD40L-CD40 interaction.
H H Jabara; R S Geha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International immunology     Volume:  8     ISSN:  0953-8178     ISO Abbreviation:  Int. Immunol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-10     Completed Date:  1997-02-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1503-10     Citation Subset:  IM    
Division of Immunology, Children's Hospital, Boston, MA, USA.
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MeSH Terms
B-Lymphocytes / drug effects,  immunology,  metabolism
Bacterial Toxins*
CD40 Ligand
Enterotoxins / pharmacology*
Immunoglobulin Class Switching / drug effects*
Immunoglobulin E / biosynthesis,  drug effects,  immunology*
Membrane Glycoproteins / biosynthesis*,  drug effects
Staphylococcus aureus / immunology
Superantigens / immunology,  pharmacology*
T-Lymphocytes / drug effects,  immunology,  metabolism
Grant Support
Reg. No./Substance:
0/Bacterial Toxins; 0/Enterotoxins; 0/Ligands; 0/Membrane Glycoproteins; 0/Superantigens; 0/enterotoxin F, Staphylococcal; 147205-72-9/CD40 Ligand; 37341-29-0/Immunoglobulin E

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