| The sulphation pattern in chondroitin sulphate chains investigated by chondroitinase ABC and ACII digestion and reactivity with monoclonal antibodies. | |
| | |
MedLine Citation:
|
PMID: 7514097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have used progressive chondroitinase digestion of pig aggrecan in conjunction with ELISA assays and disaccharide analysis to derive information about the pattern of 4- and 6-sulphation in chondroitin sulphate chains. Digestion with chondroitinase ABC resulted in the release of mainly disaccharides from the nonreducing terminal of chondroitin sulphate chains but there was also the release of some tetra- and hexa-saccharides which were degraded to disaccharides with more extensive digestion. Chondroitinase ACII, in contrast, released only disaccharides. Analysis of the disaccharide composition of the intact and digested products at different stages of digestion showed that there was a slight increase in 6-sulphate content of the chains as they were shortened. Reaction of the partially digested proteoglycans with monoclonal antibodies 3-B-3 and 3-D-5 which recognise chains terminating in 6- or 4-sulphated disaccharides, respectively, showed major differences between chondroitinase ABC and ACII products. The results suggested that chondroitinase ABC preferentially cleaved next to 4-sulphated, rather than 6-sulphated disaccharides and this resulted in some oligosaccharides as well as disaccharide being released. Chondroitinase ACII also cleaved an additional disaccharide next to the linkage to protein of chondroitin sulphate, which was not removed by chondroitinase ABC and this disaccharide was mainly nonsulphated. |
| | |
Authors:
|
T E Hardingham; A J Fosang; N J Hey; P K Hazell; W J Kee; R J Ewins |
Related Documents
:
|
20868987 - Action of propolis and medications against escherichia coli and endotoxin in root canals. 16952837 - Enhancement of styrene removal efficiency in biofilter by mixed cultures of pseudomonas... 15506197 - Reductive dechlorination of alpha-, beta-, delta-, and gamma-hexachlorocyclohexane isom... 11032487 - Selective enzyme-mediated extraction of capsaicinoids and caratenoids from chili guajil... 10581887 - Health problems and service utilization in the homeless. 20448497 - Elizabeth blackwell: a pioneer female medical graduate. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Carbohydrate research Volume: 255 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1994 Mar |
Date Detail:
|
Created Date: 1994-06-14 Completed Date: 1994-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 241-54 Citation Subset: IM |
Affiliation:
|
Biochemistry Division, Kennedy Institute of Rheumatology, Hammersmith, London, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aggrecans Animals Antibodies, Monoclonal Antibody Specificity Carbohydrate Sequence Chondroitin Lyases / metabolism Chondroitin Sulfates / chemistry, immunology*, metabolism Epitopes Extracellular Matrix Proteins* Lectins, C-Type Molecular Sequence Data Proteochondroitin Sulfates / chemistry, immunology*, metabolism Proteoglycans / chemistry*, immunology, metabolism Swine |
| Chemical | |
Reg. No./Substance:
|
0/Aggrecans; 0/Antibodies, Monoclonal; 0/Epitopes; 0/Extracellular Matrix Proteins; 0/Lectins, C-Type; 0/Proteochondroitin Sulfates; 0/Proteoglycans; 9007-28-7/Chondroitin Sulfates; EC 4.2.2.-/Chondroitin Lyases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structure of the O-specific side chain of the lipopolysaccharide from Escherichia coli O126.
Next Document: Preliminary investigation into the purification, NMR analysis, and molecular modelling of chondroiti...