| A sulfated glucosylceramide from rat kidney. | |
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MedLine Citation:
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PMID: 2925645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel sulfated glycosphingolipid containing a sulfated glucosyl residue was isolated from rat kidney and purified to homogeneity by column chromatographies with DEAE-Sephadex and silica beads. By compositional analyses, permethylation studies, one- and two-dimensional proton magnetic resonance spectroscopy, infrared spectroscopy, negative secondary ion mass spectrometry, solvolysis, and immunostaining on thin layer chromatogram, the structure of this glycolipid was proposed to be HSO3-3Glc beta 1-1Cer (where Cer is ceramide). The ceramide portion consisted of 4-D-hydroxysphinganine as the sole long chain base, and the fatty acid consisted of predominantly tetracosanoic acid, deduced from both composition analysis and negative secondary ion mass spectrometry. The yield of glucosyl sulfatide was about 5 nmol/g of tissue, being about three times as much as that of lactosylceramide sulfate. |
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Authors:
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N Iida; T Toida; Y Kushi; S Handa; P Fredman; L Svennerholm; I Ishizuka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 264 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1989 Apr |
Date Detail:
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Created Date: 1989-05-11 Completed Date: 1989-05-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5974-80 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Teikyo University, School of Medicine, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Ion Exchange Chromatography, Thin Layer Fatty Acids / analysis Glycolipids / isolation & purification Indicators and Reagents Kidney / analysis* Magnetic Resonance Spectroscopy Mass Spectrometry Methylation Rats Rats, Inbred Strains Spectrophotometry, Infrared Sulfoglycosphingolipids / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Glycolipids; 0/Indicators and Reagents; 0/Sulfoglycosphingolipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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