Document Detail


The suitability of restriction fragment length polymorphism markers for evaluating genetic diversity among and synteny between mosquito species.
MedLine Citation:
PMID:  7909414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Restriction fragment length polymorphism (RFLP) markers derived from the yellow fever mosquito, Aedes aegypti, were used in hybridizations to genomic DNA of the following mosquito species: Ae. albopictus, Ae. togoi, Armigeres subalbatus, Culex pipiens, and Anopheles gambiae. Interspecific hybridization with Ae. aegypti probes varied from 50% (An. gambiae) to 100% (Ae. albopictus) under high stringency conditions. We demonstrated the usefulness of using RFLP profiles to examine genetic diversity between mosquito populations; Ae. aegypti RFLP markers were used to examine genetic relatedness between 10 laboratory strains of Ae. aegypti as well as between nine populations representing four Cx. pipiens subspecies. These results indicate that many Ae. aegypti RFLP markers should have direct applicability in gaining a better understanding of genome structure in other mosquito species, including RFLP linkage mapping and determinations of genetic relatedness among field populations.
Authors:
D W Severson; A Mori; Y Zhang; B M Christensen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  50     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-05-26     Completed Date:  1994-05-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  425-32     Citation Subset:  AIM; IM    
Affiliation:
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern
Culicidae / genetics*
DNA / analysis
DNA Probes
Genetic Variation*
Insect Vectors / genetics*
Linkage (Genetics)
Polymorphism, Restriction Fragment Length*
Grant Support
ID/Acronym/Agency:
AI-28781/AI/NIAID NIH HHS; AI-33127/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA Probes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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