Document Detail


A subpopulation of bone marrow cells depleted by a novel antibody, anti-Liv8, is useful for cell therapy to repair damaged liver.
MedLine Citation:
PMID:  14706657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously reported a new in vivo model named as "GFP/CCl(4) model" for monitoring the transdifferentiation of green fluorescent protein (GFP) positive bone marrow cell (BMC) into albumin-positive hepatocyte under the specific "niche" made by CCl(4) induced persistent liver damage, but the subpopulation which BMCs transdifferentiate into hepatocytes remains unknown. Here we developed a new monoclonal antibody, anti-Liv8, using mouse E 11.5 fetal liver as an antigen. Anti-Liv8 recognized both hematopoietic progenitor cells in fetal liver at E 11.5 and CD45-positive hematopoietic cells in adult bone marrow. We separated Liv8-positive and Liv8-negative cells and then transplanted these cells into a continuous liver damaged model. At 4 weeks after BMC transplantation, more efficient repopulation and transdifferentiation of BMC into hepatocytes were seen with Liv8-negative cells. These findings suggest that the subpopulation of Liv8-negative cells includes useful cells to perform cell therapy on repair damaged liver.
Authors:
Naoki Yamamoto; Shuji Terai; Shinya Ohata; Tomomi Watanabe; Kaoru Omori; Koh Shinoda; Koji Miyamoto; Toshiaki Katada; Isao Sakaida; Hiroshi Nishina; Kiwamu Okita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  313     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-06     Completed Date:  2004-03-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1110-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University School of Medicine, Minami Kogushi 1-1-1, Ube, Yamaguchi 755-8505, Japan.
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MeSH Terms
Descriptor/Qualifier:
Albumins / metabolism
Animals
Antibodies, Monoclonal*
Antigens, CD45 / metabolism
Bone Marrow Cells / classification*,  immunology*
Bone Marrow Transplantation
Carbon Tetrachloride / toxicity
Cell Differentiation
Cell Separation
Female
Fetus / cytology,  immunology
Green Fluorescent Proteins
Hematopoietic Stem Cells / immunology
Hepatocytes / cytology,  immunology
Liver / immunology*,  injuries*,  metabolism
Luminescent Proteins / genetics
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Phenotype
Recombinant Proteins / genetics
Chemical
Reg. No./Substance:
0/Albumins; 0/Antibodies, Monoclonal; 0/Luminescent Proteins; 0/Recombinant Proteins; 147336-22-9/Green Fluorescent Proteins; 56-23-5/Carbon Tetrachloride; EC 3.1.3.48/Antigens, CD45

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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