Document Detail

A subcloned human esophageal squamous cell carcinoma cell line with low thrombomodulin expression showed increased invasiveness compared with a high thrombomodulin-expressing clone--thrombomodulin as a possible candidate for an adhesion molecule of squamous cell carcinoma.
MedLine Citation:
PMID:  9619877     Owner:  NLM     Status:  MEDLINE    
Thrombomodulin (TM) is an endothelial cell surface glycoprotein which converts thrombin from a procoagulant protease to an anticoagulant. We have previously reported that TM is a useful marker for immunohistochemical diagnosis of angiogenic tumors and also have reported that TM is expressed on squamous cell carcinoma (SCC) of the human esophagus. In addition, the expression of TM is significantly decreased in metastatic foci in lymph nodes compared with that in primary lesions. In order to reveal the biological significance of TM in SCC, we subcloned and established two different cell lines, i.e. TM-high-expressing (TE3HTM) cells and TM-low-expressing (TE3LTM) cells, from a human SCC cell line, TE3, using fluorescence-activated cell sorter (FACS) and examined the biological characteristics of these variant cell lines. These tumor cells revealed very similar morphological figures in ordinary cultured conditions and showed almost equal growth rates under various cultured conditions. By the invasion assay of these tumor cells using matrigel, we found that TE3LTM cells showed significantly increased invasive ability compared with that of TE3HTM cells. Characteristic intercellular localization of TM and a different manner of invasiveness between TE3LTM cells and TE3HTM cells suggest that TM may act as a cell-to-cell interaction molecule.
Y Matsushita; K Yoshiie; Y Imamura; H Ogawa; H Imamura; S Takao; S Yonezawa; T Aikou; I Maruyama; E Sato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  127     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-16     Completed Date:  1998-06-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Department of Pathology II, Faculty of Medicine, Kagoshima University, Japan.
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MeSH Terms
Carcinoma, Squamous Cell / metabolism*,  pathology*
Cell Separation
Clone Cells
Esophageal Neoplasms / metabolism*,  pathology*
Neoplasm Invasiveness*
Protein C / metabolism
Thrombomodulin / metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/Protein C; 0/Thrombomodulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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