Document Detail

A study of triplex formatin of 5'-d-T-(C-T-)2C-(T-)4C-(T-C-)2T with 5'-d-A-(G-A-)2G A hairpin triplex with three T.A.T and three C+.G.C bases triads.
MedLine Citation:
PMID:  7669269     Owner:  NLM     Status:  MEDLINE    
The UV mixing titration, gel electrophoresis, and CD measurements indicate that oligomers with a basic sequence of 5'-d-T-(C-T-)2C-(T-)4C-(T-C-)2T form a hairpin type triplex with the target 5'-d-A-(G-A-)2G. The stability, measured UV melting temperatures, were studied in aqueous solution as functions of mC (5-methylcytidine) replacement of C, pH (4 to 7), and salt concentrations (up to 1 M). The order of stability is 5'-d-A-(G-A-)2G + 5'-d-T-(C-T-)2C-(T-)4C-(T-C-)2T < 5'-d-A-(G-A-)2G + 5'-d-T-(mC-T-)2mC-(T-)4C-(T-C-)2T approximately 5'-d-A-(G-A-)2G + 5'-d-T-(C-T-)2C-(T-)4 mC-(T-mC-)2T < 5'-d-A-(G-A-)2G + 5'-d-T-(mC-T-)2mC-(T-)4mC-(T-mC-)2T at pH 4. These results indicate that (a) a stable triplex is formed with three T.A.T and three C+.G.C base triads and (b) mC is more effective than C to stablize the triplex formation in acidic condition. Thus, this provides a simple system for further studies of triplex.
L M Tsay; S B Lin; H T Tsay; H H Chen; L S Kan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomolecular structure & dynamics     Volume:  12     ISSN:  0739-1102     ISO Abbreviation:  J. Biomol. Struct. Dyn.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-10-19     Completed Date:  1995-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8404176     Medline TA:  J Biomol Struct Dyn     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1235-45     Citation Subset:  IM    
Institute of Chemistry, Academia Sinica, Taipei, Taiwan.
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MeSH Terms
Base Sequence
Cytidine / analogs & derivatives,  chemistry
Hydrogen-Ion Concentration
Molecular Sequence Data
Nucleic Acid Conformation*
Oligodeoxyribonucleotides / chemistry*
Sodium Chloride / chemistry
Structure-Activity Relationship
Reg. No./Substance:
0/Oligodeoxyribonucleotides; 2140-61-6/5-methylcytidine; 65-46-3/Cytidine; 7647-14-5/Sodium Chloride

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