| A study of triplex formatin of 5'-d-T-(C-T-)2C-(T-)4C-(T-C-)2T with 5'-d-A-(G-A-)2G A hairpin triplex with three T.A.T and three C+.G.C bases triads. | |
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MedLine Citation:
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PMID: 7669269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The UV mixing titration, gel electrophoresis, and CD measurements indicate that oligomers with a basic sequence of 5'-d-T-(C-T-)2C-(T-)4C-(T-C-)2T form a hairpin type triplex with the target 5'-d-A-(G-A-)2G. The stability, measured UV melting temperatures, were studied in aqueous solution as functions of mC (5-methylcytidine) replacement of C, pH (4 to 7), and salt concentrations (up to 1 M). The order of stability is 5'-d-A-(G-A-)2G + 5'-d-T-(C-T-)2C-(T-)4C-(T-C-)2T < 5'-d-A-(G-A-)2G + 5'-d-T-(mC-T-)2mC-(T-)4C-(T-C-)2T approximately 5'-d-A-(G-A-)2G + 5'-d-T-(C-T-)2C-(T-)4 mC-(T-mC-)2T < 5'-d-A-(G-A-)2G + 5'-d-T-(mC-T-)2mC-(T-)4mC-(T-mC-)2T at pH 4. These results indicate that (a) a stable triplex is formed with three T.A.T and three C+.G.C base triads and (b) mC is more effective than C to stablize the triplex formation in acidic condition. Thus, this provides a simple system for further studies of triplex. |
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Authors:
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L M Tsay; S B Lin; H T Tsay; H H Chen; L S Kan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomolecular structure & dynamics Volume: 12 ISSN: 0739-1102 ISO Abbreviation: J. Biomol. Struct. Dyn. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-10-19 Completed Date: 1995-10-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8404176 Medline TA: J Biomol Struct Dyn Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1235-45 Citation Subset: IM |
Affiliation:
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Institute of Chemistry, Academia Sinica, Taipei, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cytidine / analogs & derivatives, chemistry Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Conformation* Oligodeoxyribonucleotides / chemistry* Sodium Chloride / chemistry Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Oligodeoxyribonucleotides; 2140-61-6/5-methylcytidine; 65-46-3/Cytidine; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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