| A study of surface property changes in rat mesothelial cells induced by asbestos using aqueous two-phase polymer solutions. | |
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MedLine Citation:
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PMID: 7686399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intraperitoneal (i.p.) injection of crocidolite asbestos was used to induce mesotheliomas in rats. The morphological changes of the mesothelial cells were studied by light and electron microscopy and by cytologic examination of peritoneal washings. After injection, the asbestos fibres stimulated an acute inflammatory response and were rapidly phagocytosed by the mesothelial cells and incorporated into the submesothelial tissues. At 7 days, the normal microvillous surface of the mesothelium was replaced with a syncytium of proliferating mesothelial cells showing extensive loss of microvilli. Nine months or so later, multifocal mesothelial tumours arose within the peritoneal cavity. The surface thermodynamic properties of normal, asbestos-stimulated mesothelial cells and of mesothelial tumour cells in culture were studied using an aqueous two-phase system containing 4% Dextran T-2000 and 4% poly (ethylene glycol) (PEG) w/w. After asbestos stimulation, there was a significant (P < 0.01) increase in contact angle between the dextran-rich phase and the mesothelial cell surface. These changes were even greater for the mesothelial tumours. The results indicate that the work of adhesion for asbestos-stimulated mesothelial cells and mesothelial tumours is lower than in normal tissue. These findings may be relevant to the process of tumour spread in the serosal cavities and to the development of distant metastases. |
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Authors:
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M M Lee; F H Green; D J Demetrick; X Jiang X; S Schürch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1181 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-08-03 Completed Date: 1993-08-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 223-32 Citation Subset: IM |
Affiliation:
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Department of Medical Physiology, University of Calgary, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asbestos / administration & dosage, toxicity* Cell Adhesion Cell Transformation, Neoplastic / chemistry, ultrastructure* Dextrans Epithelium / chemistry, ultrastructure Male Mesothelioma / chemistry, etiology*, ultrastructure Microscopy, Electron, Scanning Peritoneal Neoplasms / chemistry, etiology*, ultrastructure Polyethylene Glycols Rats Rats, Inbred F344 Surface Properties Thermodynamics Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Polyethylene Glycols; 1332-21-4/Asbestos; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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