Document Detail

A study of some practical aspects of high temperature liquid chromatography in pharmaceutical applications.
MedLine Citation:
PMID:  20934825     Owner:  NLM     Status:  In-Process    
In the pharmaceutical industry fast and efficient separation techniques play an increasing role among analytical methods because the samples to be investigated grow both in complexity and number, and there is an increasing time pressure to complete the analysis. Reducing the analysis time without decreasing the efficiency is possible using higher pressures, elevated temperatures, smaller particle sizes, or a combination of these approaches. Recently developed chromatographic techniques such as the UHPLC (ultra high performance liquid chromatography) and HTLC (high temperature liquid chromatography) are highly promising in meeting these demands. In this study, high temperature liquid chromatography (HTLC) with a zirconia-based column and temperatures elevated up to 150°C was used. We investigated the chromatographic behaviour of a steroid active pharmaceutical ingredient (levonorgestrel) and its structurally related impurities as model compounds. The effect of the temperature in the range of 50-150°C and the flow-rate in the range of 0.5-3.0 ml/min, and using methanol as an organic modifier, were studied for optimisation of the separation method.
Renáta Berta; Mónika Babják; Mária Gazdag
Related Documents :
25288885 - Limitations of using propidium monoazide with qpcr to discriminate between live and dea...
25405705 - Highly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic...
2016385 - Determination of beta-carbolines in foodstuffs by high-performance liquid chromatograph...
20934825 - A study of some practical aspects of high temperature liquid chromatography in pharmace...
24248825 - Continuous flow fast atom bombardment with packed microcolumns: a comparison of precolu...
23813595 - Quantitative determination of d- and l-threo enantiomers of methylphenidate in brain ti...
Publication Detail:
Type:  Journal Article     Date:  2010-09-17
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  54     ISSN:  1873-264X     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2010-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  458-62     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Chemical Works of Gedeon Richter Plc., P.O. Box 27, Gyömrői út 19-21, 1103 Budapest 10, Hungary.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification, isolation and characterization of a new degradation product in sultamicillin drug su...
Next Document:  Electrocardiogram and echocardiogram findings in runners completing a half marathon.