| A study of the relationship between pharmacologic preconditioning and adenosine triphosphate-sensitive potassium (KATP) channels on cultured cardiomyocytes using the microelectrode array. | |
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MedLine Citation:
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PMID: 20648957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The microelectrode array was used to study the pharmacologic preconditioning effect of adenosine triphosphate-sensitive channel activation using potassium channel openers (KCOs) on rat cardiomyocytes over 90 minutes of ischemia. Cell viability and electrophysiological changes between KCOs pretreated and untreated cardiomyocytes were compared. Ischemia caused significant increases in beat frequency, extracellular field potential amplitude, and propagation velocity of spontaneously beating untreated cardiomyocytes. However, these electrophysiological parameters reduced as the duration of ischemia increased. The electrophysiological changes on ischemic cardiomyocytes were abolished by pretreating the cells with KCOs. Pinacidil pretreated cardiomyocytes retained a significantly higher viability than the untreated cardiomyocytes after 90 minutes of ischemia. Because Connexin 43 has a direct correlation with the propagation velocity, the Connexin 43 protein expression was also investigated. Connexin 43 expression levels were lower in KCOs pretreated cardiomyocytes than that of the untreated controls, and this correlated with the propagation velocity results obtained from the microelectrode array. The effect of pinacidil (sarcolemmal adenosine triphosphate-sensitive channel opener) was more prominent than that of diazoxide (mitochondrial K adenosine triphosphate-sensitive channel opener) on ischemic cardiomyocytes as indicated in the present acute ischemia study. |
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Authors:
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Jessica Ka-Yan Law; Chi-Kong Yeung; Ka-Lap Yiu; John Anthony Rudd; Sven Ingebrandt; Mansun Chan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cardiovascular pharmacology Volume: 56 ISSN: 1533-4023 ISO Abbreviation: J. Cardiovasc. Pharmacol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-20 Completed Date: 2010-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902492 Medline TA: J Cardiovasc Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 60-8 Citation Subset: IM |
Affiliation:
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Bioengineering Graduate Program, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Hong Kong. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Cells, Cultured Connexin 43 / genetics Diazoxide / pharmacology Electrophysiology Gene Expression Regulation / drug effects Ischemic Preconditioning, Myocardial / methods* KATP Channels / drug effects, metabolism* Microelectrodes Myocytes, Cardiac / drug effects, metabolism* Pinacidil / pharmacology Potassium Channels / drug effects, metabolism Rats Rats, Sprague-Dawley Vasodilator Agents / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/KATP Channels; 0/Potassium Channels; 0/Vasodilator Agents; 0/mitochondrial K(ATP) channel; 364-98-7/Diazoxide; 85371-64-8/Pinacidil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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