Document Detail


The study of nonspecific internalization of cholesteryl esters by the Hep G2 hepatoma cells.
MedLine Citation:
PMID:  11093583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cholesteryl oleate-POPC dispersions (1:3, mol/mol, mean particle size 110+/-20 nm) were taken up by the human hepatoma line Hep G2 cells via endocytosis. Internalization of the cholesteryl oleate-POPC dispersions by Hep G2 cells was dependent on the incubation time and dispersion concentration. At the cholesteryl oleate concentration 100 microM, its total uptake and internalization were found to be 1.5 nmol and 0.8 nmol per 1 mg of cell protein/24 h, respectively. Intracellular cleavage of the cholesteryl oleate incorporated in dispersions resulted in accumulation of free cholesterol capable of being released into the medium and metabolized to water-soluble polar products, presumably bile acids; oleic acid released is, apparently, involved in biosynthesis of triacylglycerides. The low-density lipoprotein receptor is not involved in internalization of lipid dispersions, and the presence of the cholesteryl oleate-POPC dispersions has no effect on the receptor-dependent internalization of cholesteryl esters of the low-density lipoproteins. The obtained data allow us to consider nonspecific internalization of cholesteryl esters by hepatocytes as a substantial part of the nonpolar lipid clearance.
Authors:
A F Kisseleva; N V Medvedeva; L E Goryunova; Misharin AYu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Membrane & cell biology     Volume:  14     ISSN:  1023-6597     ISO Abbreviation:  Membr Cell Biol     Publication Date:  2000  
Date Detail:
Created Date:  2001-02-14     Completed Date:  2001-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9517472     Medline TA:  Membr Cell Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  205-17     Citation Subset:  IM    
Affiliation:
Institute of Experimental Cardiology, Cardiology Research Center Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Carcinoma, Hepatocellular / metabolism*
Cholesterol Esters / metabolism*
Humans
Liver Neoplasms / metabolism*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Cholesterol Esters

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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