Document Detail


A study of the mechanism of in vitro cytotoxicity of metal oxide nanoparticles using catfish primary hepatocytes and human HepG2 cells.
MedLine Citation:
PMID:  21851965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nanoparticles (NPs), including nanometal oxides, are being used in diverse applications such as medicine, clothing, cosmetics and food. In order to promote the safe development of nanotechnology, it is essential to assess the potential adverse health consequences associated with human exposure. The liver is a target site for NP toxicity, due to NP accumulation within it after ingestion, inhalation or absorption. The toxicity of nano-ZnO, TiO(2), CuO and Co(3)O(4) was investigated using a primary culture of channel catfish hepatocytes and human HepG2 cells as in vitro model systems for assessing the impact of metal oxide NPs on human and environmental health. Some mechanisms of nanotoxicity were determined by using phase contrast inverted microscopy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, reactive oxygen species (ROS) assays, and flow cytometric assays. Nano-CuO and ZnO showed significant toxicity in both HepG2 cells and catfish primary hepatocytes. The results demonstrate that HepG2 cells are more sensitive than catfish primary hepatocytes to the toxicity of metal oxide NPs. The overall ranking of the toxicity of metal oxides to the test cells is as follows: TiO(2)<Co(3)O(4)<ZnO<CuO. The toxicity is due not only to ROS-induced cell death, but also to damages to cell and mitochondrial membranes.
Authors:
Yonggang Wang; Winfred G Aker; Huey-min Hwang; Clement G Yedjou; Hongtao Yu; Paul B Tchounwou
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-08-17
Journal Detail:
Title:  The Science of the total environment     Volume:  409     ISSN:  1879-1026     ISO Abbreviation:  Sci. Total Environ.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-03     Completed Date:  2012-01-23     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  0330500     Medline TA:  Sci Total Environ     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4753-62     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Department of Biology, Jackson State University, Jackson, MS, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Catfishes / metabolism*
Cell Line
Environmental Health
Flow Cytometry
Hepatocytes / drug effects*,  metabolism
Humans
Metals, Heavy / toxicity*
Nanoparticles / toxicity*
Oxides / toxicity*
Reactive Oxygen Species / metabolism
Tetrazolium Salts
Thiazoles
Grant Support
ID/Acronym/Agency:
G12 RR013459-13/RR/NCRR NIH HHS; G12RR013459-13/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Metals, Heavy; 0/Oxides; 0/Reactive Oxygen Species; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue
Comments/Corrections

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