Document Detail

A study on inhibition mechanism of breast cancer cells by bis-type triaziquone.
MedLine Citation:
PMID:  20371239     Owner:  NLM     Status:  MEDLINE    
The objectives of this study were to evaluate the antiproliferation effect of a synthetic quinone-containing compound bis-type triaziquone (BTZQ) on breast cancer cells BC-M1 and MCF-7. At a dose of 0.42 and 0.79 microM, BTZQ showed a 50% inhibition on BC-M1 and MCF-7 cell growth after 24 h treatment, respectively, but reduced to 0.2 and 0.61 microM after 48 h. A low toxic effect was observed for skin fibroblast cell after BTZQ treatment for 48 h at a dose from 0.0625-0.25 microM. BTZQ was more effective in inhibiting growth of breast cancer cells than tamoxifen. Additionally, BTZQ-treated BC-M1 cells under hypoxia condition for 48 h exhibited a higher cytotoxicity than under aerobic condition. Cell cycle analysis revealed the arrest of BC-M1 cells at G2/M phase, with accumulation of apoptotic cells at the sub-G1 phase being enhanced following a rise in dose. The expression levels of caspase-3, caspase-8 and caspase-9 were elevated in both dose- and time-dependent responses. Western blot analysis indicated that BTZQ may up-regulate expression of cyclin B, p21, p53 and cytochrome c, but down-regulate cdk1 expression in a dose-dependent manner, leading to apoptosis of BC-M1 cells. All these results suggested that BTZQ may be a potential anti-breast cancer drug.
Yuh-Ling Lin; Yi-Ting Su; Bing-Huei Chen
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Publication Detail:
Type:  Journal Article     Date:  2010-04-02
Journal Detail:
Title:  European journal of pharmacology     Volume:  637     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
School of Medicine, Fu Jen University, Taipei, Taiwan.
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MeSH Terms
Antineoplastic Agents, Alkylating / pharmacology*
Apoptosis / drug effects
Blotting, Western
Breast Neoplasms / enzymology,  pathology*
Caspase 3 / metabolism
Caspase 8 / metabolism
Caspase 9 / metabolism
Cell Division / drug effects
Cell Line, Tumor
Dose-Response Relationship, Drug
G2 Phase / drug effects
Time Factors
Triaziquone / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents, Alkylating; 68-76-8/Triaziquone; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9

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