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A study of immunoglobulin structure. 3. An estimate of the variability of human light chains.
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MedLine Citation:
PMID:  4183485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
102 human Bence Jones proteins have been purified by gel filtration, digested with trypsin, and analyzed by peptide mapping. In several cases Bence Jones "fragments", corresponding to the variable half of the corresponding proteins, were observed. The peptide maps of the proteins were compared to establish whether any identical proteins were present in the sample analyzed. No Bence Jones protein showed a peptide map identical to that of any other protein, although remarkable similarities in the peptide maps were observed for some proteins. Two proteins that gave very similar peptide maps were then examined in detail, by purifying and analyzing the tryptic peptides. It was then found that these two proteins differ in amino acid sequence in at least six positions. The probability of not finding two identical sequences by examining a sample extracted from populations of light chains of different sizes has been calculated. This has led to an estimate of the minimal size of the population of light chain sequences in humans. The number of light chain sequences appears to be at least a few thousand. Information on the frequency of Inv and Oz antigenic determinants and on the relative frequency of subtypes of K chains has been obtained. Proteins of KI subtype are found most frequently. The possibility that different subtypes may be predominant in different species is discussed in relation to the evolutionary arguments used in favor of the somatic theories on the origin of variability of immunoglobulin chains.
Authors:
R Quattrocchi; D Cioli; C Baglioni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  130     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1969 Aug 
Date Detail:
Created Date:  1969-09-03     Completed Date:  1969-09-03     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-15     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence*
Animals
Bence Jones Protein* / analysis
Chemical Phenomena
Chemistry
Chromatography
Chromatography, Gel
Genetics, Population
Methods
Peptides / analysis
Species Specificity
Trypsin
gamma-Globulins* / analysis
Chemical
Reg. No./Substance:
0/Peptides; 0/gamma-Globulins; 9006-99-9/Bence Jones Protein; EC 3.4.21.4/Trypsin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1969 by The Rockefeller University Press
Received Day: 23 Month: 3 Year: 1969
Print publication date: Day: 1 Month: 8 Year: 1969
Volume: 130 Issue: 2
First Page: 401 Last Page: 415
ID: 2138677
PubMed Id: 4183485

A STUDY OF IMMUNOGLOBULIN STRUCTURE : III. AN ESTIMATE OF THE VARIABILITY OF HUMAN LIGHT CHAINS
R. Quattrocchi
D. Cioli
C. Baglioni
From the International Laboratory of Genetics and Biophysics, Naples, Italy, and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139


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