| A QM/MM study on the fast photocycle of blue light using flavin photoreceptors in their light-adapted/active form. | |
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MedLine Citation:
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PMID: 20532291 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Based on QM/MM calculations using a combination of time-dependent Hartree-Fock and coupled cluster response theory a mechanism is proposed for the photocycle of blue light using flavin (BLUF) domains in the signaling/light adapted conformation. In analogy to the dark-adapted form, a charge transfer state drives proton transfer from the highly conserved tyrosine residue to the flavin chromophore. The latter step is mediated by the adjacent glutamine residue, which, in the light adapted conformation, exists as its imidic tautomer. However, before the proton transfer is even halfway completed, a conical intersection seam between the charge transfer and ground state is reached. Two channels for the decay back to the initial light-adapted conformation are open, a rapid one leading directly through the funnel of the conical intersection, bypassing the formation of the biradical intermediate, and a slower one via the biradical intermediate. The mechanism as proposed here: (i) explains the very rapid photocycle; and (ii) confirms the concept of photoirreversibility, both of which have been experimentally observed for BLUF domains in their light-adapted conformations. |
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Authors:
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Keyarash Sadeghian; Marco Bocola; Martin Schütz |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-08 |
Journal Detail:
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Title: Physical chemistry chemical physics : PCCP Volume: 12 ISSN: 1463-9084 ISO Abbreviation: Phys Chem Chem Phys Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-29 Completed Date: 2010-12-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888160 Medline TA: Phys Chem Chem Phys Country: England |
Other Details:
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Languages: eng Pagination: 8840-6 Citation Subset: IM |
Affiliation:
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Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitätsstrasse 31, D-93040 Regensburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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chemistry* Darkness Flavins / chemistry* Glutamine / chemistry Light* Photoreceptors, Microbial / chemistry* Protein Structure, Tertiary Protons Quantum Theory Tyrosine / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Flavins; 0/Photoreceptors, Microbial; 0/Protons; 55520-40-6/Tyrosine; 56-85-9/Glutamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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