Document Detail

A study of cow's milk containing high levels of linoleic acid: isolation and properties of the fat-globule membrane.
MedLine Citation:
PMID:  993404     Owner:  NLM     Status:  MEDLINE    
Properties of whole milk and milk fractions from cows fed a diet that gave a greatly increased proportion of unsaturated fatty acid residues (especially of linoleic acid) in the milk lipids were studied, and this milk (high-linoleic milk) was compared with milk from cows on a control diet (control milk). The milk fractions were isolated by high-speed centrifugation of whole milk or cream and were examined by chemical analysis and electron microscopy. During centrifugation the globules of milk fat were disrupted and the membranes (fat-globule 'ghosts') floated as a layer beneath the free lipid. Membrane proteins from the 2 sorts of milk gave the same electrophoretic pattern and the amino acid compositions were the same. Lipid analysis of the membrane fraction from high-linoleic milk showed the expected increase in the proportion of unsaturated fatty acid residues in the neutral lipids, but there was an unexpected decrease in the proportion of unsaturated residues in the membrane phospholipids. No differences were found between high-linoleic and control milk in the ultrastructure of the milk-fat globules or the isolated membranes.
R W Sleigh; J M Bain; R W Burley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of dairy research     Volume:  43     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1977-01-29     Completed Date:  1977-01-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  389-400     Citation Subset:  IM    
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MeSH Terms
Amino Acids
Chemical Phenomena
Dietary Fats
Fatty Acids
Linoleic Acids*
Lipids* / analysis
Membrane Lipids*
Membrane Proteins* / isolation & purification
Membranes / analysis
Microscopy, Electron
Milk* / analysis,  cytology
Milk Proteins
Reg. No./Substance:
0/Amino Acids; 0/Dietary Fats; 0/Fatty Acids; 0/Linoleic Acids; 0/Lipids; 0/Membrane Lipids; 0/Membrane Proteins; 0/Milk Proteins

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